TY - JOUR
T1 - Intratumoral lymphatic vascular density is an independent factor for disease-free and overall survival in advanced stage oral squamous cell carcinoma
AU - Jardim, Juscelino Freitas
AU - Galvis, Marisol Miranda
AU - Fabelo, Ivete Rodrigues
AU - Soares, Fernando Augusto
AU - Pinto, Clóvis Antônio Lopes
AU - Kowalski, Luiz Paulo
N1 - Funding Information:
This work was supported by grants from the Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Brazil and the Coordination for the Improvement of Higher Education Personnel (CAPES-Brazil).
Publisher Copyright:
© 2021 Elsevier Inc.
PY - 2021/11
Y1 - 2021/11
N2 - Objective: The presence of lymphatic and blood vessels in oral squamous cell carcinoma (OSCC) should play a key role in progression and dissemination. This study aimed to evaluate the correlation between the lymphatic and blood vessel densities with prognostic outcomes in advanced stage OSCC. Study Design: Immunohistochemical reactions for D-240, CD34, and CD105 were performed in 88 advanced stage OSCC cases located at the oral tongue and the floor of the mouth. The lymphatic vascular density (LVD), blood vascular density (BVD), and neoformed vascular density (NVD) were assessed by counting positive reactions in 4 hotspot areas, both intratumoral (IT) and peritumoral (PT), at high magnification (× 40). Results: High IT LVD was associated with extracapsular spread of lymph node metastasis (P = .03). Recurrence rates were correlated with IT LVD (P < .0001), IT BVD (P = .036), and IT NVD (P = .047), and overall survival was associated with high IT LVD (P = .0016) and IT NVD (P = .009). Yet, IT LVD was an independent factor for disease-free survival and for overall survival based on the Cox proportional hazards model. Conclusions: Our results suggest that high IT LVD has a strong impact on survival outcomes in advanced stage OSCC.
AB - Objective: The presence of lymphatic and blood vessels in oral squamous cell carcinoma (OSCC) should play a key role in progression and dissemination. This study aimed to evaluate the correlation between the lymphatic and blood vessel densities with prognostic outcomes in advanced stage OSCC. Study Design: Immunohistochemical reactions for D-240, CD34, and CD105 were performed in 88 advanced stage OSCC cases located at the oral tongue and the floor of the mouth. The lymphatic vascular density (LVD), blood vascular density (BVD), and neoformed vascular density (NVD) were assessed by counting positive reactions in 4 hotspot areas, both intratumoral (IT) and peritumoral (PT), at high magnification (× 40). Results: High IT LVD was associated with extracapsular spread of lymph node metastasis (P = .03). Recurrence rates were correlated with IT LVD (P < .0001), IT BVD (P = .036), and IT NVD (P = .047), and overall survival was associated with high IT LVD (P = .0016) and IT NVD (P = .009). Yet, IT LVD was an independent factor for disease-free survival and for overall survival based on the Cox proportional hazards model. Conclusions: Our results suggest that high IT LVD has a strong impact on survival outcomes in advanced stage OSCC.
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U2 - 10.1016/j.oooo.2021.07.014
DO - 10.1016/j.oooo.2021.07.014
M3 - Article
AN - SCOPUS:85114699586
SN - 2212-4403
VL - 132
SP - 580
EP - 588
JO - Oral Surgery Oral Medicine and Oral Pathology
JF - Oral Surgery Oral Medicine and Oral Pathology
IS - 5
ER -