TY - JOUR
T1 - Investigation of foreign materials in gingival lesions
T2 - a clinicopathologic, energy-dispersive microanalysis of the lesions and in vitro confirmation of pro-inflammatory effects of the foreign materials
AU - Ferreira, Leticia
AU - Peng, Hsin Hsin
AU - Cox, Darren P.
AU - Chambers, David W.
AU - Bhula, Avni
AU - Young, John D.
AU - Ojcius, David M.
AU - Ramos-Junior, Erivan S.
AU - Morandini, Ana Carolina
N1 - Funding Information:
This work was supported by Interdisciplinary Pilot Project Research Award DRES03- Activity 116, 2017 and DRES03-Activity 117, 2017 are intramural grants from the University of the Pacific Arthur A. Dugoni School of Dentistry and by funds from Primordia Institute of New Sciences and Medicine and by grants from Chang Gung University (QZRPD146), Ming Chi University of Technology (0 XB0), and Chang Gung Memorial Hospital (CMRPG3 F0772).
Funding Information:
This work was supported by Interdisciplinary Pilot Project Research Award DRES03- Activity 116, 2017 and DRES03-Activity 117, 2017 are intramural grants from the University of the Pacific Arthur A. Dugoni School of Dentistry and by funds from Primordia Institute of New Sciences and Medicine and by grants from Chang Gung University (QZRPD146), Ming Chi University of Technology (0 XB0), and Chang Gung Memorial Hospital (CMRPG3 F0772).We thank the Microscopy Center of the Chang Gung University, Taiwan, for use of the facility and for providing technical support.
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/9
Y1 - 2019/9
N2 - Objectives: This study aimed to evaluate the clinical and histopathologic features of gingival lesions containing foreign material (GLFMs). In parallel, the composition of the foreign material and its effects in primary human gingival fibroblasts (HGFs) were investigated. Study Design: Eighty-six GLFMs were retrieved from an oral pathology biopsy service. Clinical and microscopic data were analyzed, and the composition of the particles was identified by using energy-dispersive X-ray spectroscopy (EDX). Furthermore, HGFs were stimulated with silica (SiO2) microparticles to investigate the production of collagen type 1 (COL-1), matrix metalloproteinase 2 (MMP2), and inflammatory cytokines. Results: GLFMs were most commonly found in women (60.5%) and most frequently described as white plaques. Histopathologic examination identified verrucous hyperplasia in 59% and epithelial dysplasia in 28% of the cases. EDX microanalysis revealed that Si (94%) was the most frequently detected foreign element. SiO2 microparticles induced higher COL-1 expression; higher levels of proinflammatory cytokines, such as interleukin-6 (IL-6), IL-8, and transforming growth factor-β, and increased MMP-2 activity in HGFs. Conclusions: There was a strong association between the presence of foreign material in the gingiva and white verrucous clinical lesions. In addition, the most common element in the foreign material was Si, and our in vitro findings demonstrate the importance of silica-mediated effects on gingival fibroblasts.
AB - Objectives: This study aimed to evaluate the clinical and histopathologic features of gingival lesions containing foreign material (GLFMs). In parallel, the composition of the foreign material and its effects in primary human gingival fibroblasts (HGFs) were investigated. Study Design: Eighty-six GLFMs were retrieved from an oral pathology biopsy service. Clinical and microscopic data were analyzed, and the composition of the particles was identified by using energy-dispersive X-ray spectroscopy (EDX). Furthermore, HGFs were stimulated with silica (SiO2) microparticles to investigate the production of collagen type 1 (COL-1), matrix metalloproteinase 2 (MMP2), and inflammatory cytokines. Results: GLFMs were most commonly found in women (60.5%) and most frequently described as white plaques. Histopathologic examination identified verrucous hyperplasia in 59% and epithelial dysplasia in 28% of the cases. EDX microanalysis revealed that Si (94%) was the most frequently detected foreign element. SiO2 microparticles induced higher COL-1 expression; higher levels of proinflammatory cytokines, such as interleukin-6 (IL-6), IL-8, and transforming growth factor-β, and increased MMP-2 activity in HGFs. Conclusions: There was a strong association between the presence of foreign material in the gingiva and white verrucous clinical lesions. In addition, the most common element in the foreign material was Si, and our in vitro findings demonstrate the importance of silica-mediated effects on gingival fibroblasts.
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U2 - 10.1016/j.oooo.2019.04.009
DO - 10.1016/j.oooo.2019.04.009
M3 - Article
C2 - 31300373
AN - SCOPUS:85068480421
SN - 2212-4403
VL - 128
SP - 250
EP - 267
JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
IS - 3
ER -