Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Jun Jie Wang, Xiu Qing Fu, Yu Guang Guo, Lin Yuan, Qian Qian Gao, Hua Li Yu, Heng Liang Shi, Xing Zhi Wang, Wencheng Xiong, Xiao Juan Zhu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

Original languageEnglish (US)
Pages (from-to)578-585
Number of pages8
JournalCell Biology International
Volume33
Issue number5
DOIs
StatePublished - May 1 2009

Fingerprint

Myosins
Gonadotropin-Releasing Hormone
Focal Adhesions
Brain

Keywords

  • Adhesion
  • Filopodia
  • Migration
  • Overexpression

ASJC Scopus subject areas

  • Cell Biology

Cite this

Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells. / Wang, Jun Jie; Fu, Xiu Qing; Guo, Yu Guang; Yuan, Lin; Gao, Qian Qian; Yu, Hua Li; Shi, Heng Liang; Wang, Xing Zhi; Xiong, Wencheng; Zhu, Xiao Juan.

In: Cell Biology International, Vol. 33, No. 5, 01.05.2009, p. 578-585.

Research output: Contribution to journalArticle

Wang, JJ, Fu, XQ, Guo, YG, Yuan, L, Gao, QQ, Yu, HL, Shi, HL, Wang, XZ, Xiong, W & Zhu, XJ 2009, 'Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells', Cell Biology International, vol. 33, no. 5, pp. 578-585. https://doi.org/10.1016/j.cellbi.2009.02.006
Wang, Jun Jie ; Fu, Xiu Qing ; Guo, Yu Guang ; Yuan, Lin ; Gao, Qian Qian ; Yu, Hua Li ; Shi, Heng Liang ; Wang, Xing Zhi ; Xiong, Wencheng ; Zhu, Xiao Juan. / Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells. In: Cell Biology International. 2009 ; Vol. 33, No. 5. pp. 578-585.
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