Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells

Jun Jie Wang, Xiu Qing Fu, Yu Guang Guo, Lin Yuan, Qian Qian Gao, Hua Li Yu, Heng Liang Shi, Xing Zhi Wang, Wen Cheng Xiong, Xiao Juan Zhu

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.

Original languageEnglish (US)
Pages (from-to)578-585
Number of pages8
JournalCell Biology International
Volume33
Issue number5
DOIs
StatePublished - May 2009

Keywords

  • Adhesion
  • Filopodia
  • Migration
  • Overexpression

ASJC Scopus subject areas

  • Cell Biology

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