Ionomycin restores taurine transporter activity in cyclosporin-a treated macrophages

Ha Won Kim, Eun Jin Lee, Won Bae Kim, Byong Kak Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Taurine is accumulated at high concentrations in various tissues. The taurine transporter (TAUT) is responsible for the transportation of taurine in the cell. The transporter is affected by various stimuli to maintain cell volume. Macrophage cell volume varies in its activated states. In our experiment, it was found that the murine macrophage cell line, RAW264.7, expressed TAUT protein in its membrane. Its transporting activities could be blocked by β-amino acid such as β-alanine, but not by α-amino acids in this cell line, when assessed in RAW264.7 cells under the influence of immunosuppressive reagents, the activity of the TAUT was decreased by treatment with rapamycin (RM) or cyclosporin A (CSA). However, when ionomycin (IM) was added to this system, TAUT activity was recovered only in CSA-treated cells, in a concentration-dependent manner. in order to inhibit voltage gated Ca2+ channels, calmidazolium was added to the RAW264.7 cell line. Treatment of the cells with calmidazolium completely blocked TAUT. Furthermore, addition of IM to this system resulted in recovery the activity of TAUT again. When we added phorbol myristyl acetate (PMA) to the cell line, secretion of nitric oxide (NO) was increased 4-fold and the TAUT activity was decreased 5-fold. However, the addition of N-nitro L-arginine methyl ester (L-NAME), an inducible NO synthase (iNOS) inhibitor, to the PMA-treated cells induced recovery of TAUT activity. These results showed that the activity of TAUT was sensitive to both the intracellular concentrations of Ca2+ and NO.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalAdvances in Experimental Medicine and Biology
Volume483
StatePublished - Dec 1 2000
Externally publishedYes

Fingerprint

Ionomycin
Macrophages
Cyclosporine
Cells
calmidazolium
Cell Line
Taurine
Cell Size
Nitric Oxide
Acetates
taurine transporter
Amino Acids
Recovery
Arginine
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase Type II
Sirolimus
Immunosuppressive Agents
Alanine
Esters

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ionomycin restores taurine transporter activity in cyclosporin-a treated macrophages. / Kim, Ha Won; Lee, Eun Jin; Kim, Won Bae; Kim, Byong Kak.

In: Advances in Experimental Medicine and Biology, Vol. 483, 01.12.2000, p. 127-135.

Research output: Contribution to journalArticle

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