Irisolidone, an isoflavone metabolite, represses JC virus gene expression via inhibition of Sp1 binding in human glial cells

So Young Kim, Dong Hyun Kim, Jin Won Hyun, John W. Henson, Hee Sun Kim

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Progressive multifocal leukoencephalopathy (PML) is a fatal demyelinating disease that results from an oligodendrocyte infection caused by the JC virus. Therefore, inhibiting the expression of JC virus is important for preventing and/or treating PML. This study found that irisolidone, an isoflavone metabolite, significantly inhibited the JC virus expression in primary cultured human astrocytes and glial cell lines. Studies examining the underlying mechanism revealed that a mutation of the Sp1 binding site downstream of the TATA box (Sp1-II) dramatically diminished the inhibitory activity of irisolidone. In addition, an irisolidone treatment repressed Sp1 binding to Sp1-II site, which is important for the basal JC virus promoter activity. The results suggest that the inhibitory effect of irisolidone against the JC virus may be attributed at least in part to the suppression of Sp1 binding to the JC virus promoter region. Therefore, the inhibition of the JC virus expression by irisolidone might provide therapeutic potential for PML caused by the JC virus.

Original languageEnglish (US)
Pages (from-to)3-8
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number1
DOIs
StatePublished - May 26 2006
Externally publishedYes

Keywords

  • Gene expression
  • Irisolidone
  • JC virus
  • Progressive multifocal leukoencephalopathy
  • Promoter
  • Sp1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Irisolidone, an isoflavone metabolite, represses JC virus gene expression via inhibition of Sp1 binding in human glial cells'. Together they form a unique fingerprint.

Cite this