Iron attenuates nitric oxide level and iNOS expression in endotoxin-treated mice

Andrei M. Komarov, David L. Mattson, I. Tong Mak, William B. Weglicki

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The effect of exogenous Fe-citrate complex (Fe doses of 120 and 230 μmol/kg) on nitric oxide (NO) production in vivo has been studied in blood and liver tissue of endotoxin-treated mice. Fe-citrate complex was administered to mice subcutaneously at the same time with intravenous injection of Escherichia coli lipopolysaccharide (LPS). Iron-dependent decrease in NO2-/NO3- and nitrosyl hemoglobin levels in blood of animals was detected at 6 h after LPS administration, suggesting systemic attenuation of NO generation. NO production in the liver tissue of LPS-treated mice was decreased after Fe administration judging from the amount of mononitrosyl-iron complexes formed in the tissue by diethyldithiocarbamate. The iNOS protein determination in the liver tissue of LPS-treated mice demonstrated iron-dependent inhibition of iNOS expression. We have found previously that exogenous iron does not affect systemic NO level when it is given at 6 h after LPS injection, i.e. after iNOS expression. This is a first report demonstrating iron-dependent iNOS down-regulation in endotoxin-treated mice.

Original languageEnglish (US)
Pages (from-to)253-256
Number of pages4
JournalFEBS Letters
Volume424
Issue number3
DOIs
StatePublished - Mar 13 1998
Externally publishedYes

Keywords

  • Inducible nitric oxide synthase
  • Iron
  • Nitric oxide
  • Nitrosyl iron complex
  • Septic shock
  • Spin trapping

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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