Is the inactivation of dentin proteases by crosslinkers reversible?

Roda Seseogullari-Dirihan, Mustafa Murat Mutluay, David Henry Pashley, Arzu Tezvergil-Mutluay

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective Inactivation of dentin proteases by crosslinkers has been suggested as a way to prevent the degradation of dentin collagen in the hybrid layer. However, it is not known if the inhibition is reversible. The aim of this study was to evaluate the inactivation effect of various crosslinkers on dentin protease activity over a period of 6 months. Methods Demineralized dentin beams (1 × 2 × 6 mm, n = 10/group) were treated with (1) 1% glutaraldehyde (GA1), (2) 5% glutaraldehyde (GA5), (3) 1% grape seed extract (GS1), (4) 5% grape seed extract (GS5), (5) 10% sumac berry extract (S), (6) 20 μM curcumin (CR20), and (7) 200 μM curcumin (CR200) for 5 min. Untreated beams served as control. The beams were incubated up to 6 months and incubation media were used to analyze solubilized telopeptide (ICTP and CTX) fragments as indicators of MMP- and cathepsin K-mediated degradation after 1, 3 and 6 months of incubation. The relative MMP activity of dentin beams was tested using a generic MMP assay. Data were analyzed using repeated-measures ANOVA, α = 0.05. Results All treated groups showed significant decrease in CTX release (32.2–469.5 pg/mg dentin) and ICTP (1.8–47.6 ng/mg dentin) fragments during the first month of incubation compared to control (1159 pg/mg and 72.9 ng/mg dentin, respectively). GA5, GS5 and CR200 maintained their inhibitory effect during 6-month incubation. The results were confirmed by dry mass loss and relative MMP activity following 6 months. Significance The results of this study indicate that the long-term effect is both crosslinker and dose dependent.

Original languageEnglish (US)
Pages (from-to)e62-e68
JournalDental Materials
Volume33
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Curcumin
Dentin
Matrix Metalloproteinases
Seed
Peptide Hydrolases
Grape Seed Extract
Degradation
Glutaral
Analysis of variance (ANOVA)
Collagen
Assays
Cathepsin K
Rhus
Fruit
Analysis of Variance
Cathepsins

Keywords

  • Crosslinkers
  • Cysteine cathepsins
  • Degradation
  • Dentin
  • Matrix metalloproteinases

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Seseogullari-Dirihan, R., Mutluay, M. M., Pashley, D. H., & Tezvergil-Mutluay, A. (2017). Is the inactivation of dentin proteases by crosslinkers reversible? Dental Materials, 33(2), e62-e68. https://doi.org/10.1016/j.dental.2016.09.036

Is the inactivation of dentin proteases by crosslinkers reversible? / Seseogullari-Dirihan, Roda; Mutluay, Mustafa Murat; Pashley, David Henry; Tezvergil-Mutluay, Arzu.

In: Dental Materials, Vol. 33, No. 2, 01.02.2017, p. e62-e68.

Research output: Contribution to journalArticle

Seseogullari-Dirihan, R, Mutluay, MM, Pashley, DH & Tezvergil-Mutluay, A 2017, 'Is the inactivation of dentin proteases by crosslinkers reversible?', Dental Materials, vol. 33, no. 2, pp. e62-e68. https://doi.org/10.1016/j.dental.2016.09.036
Seseogullari-Dirihan R, Mutluay MM, Pashley DH, Tezvergil-Mutluay A. Is the inactivation of dentin proteases by crosslinkers reversible? Dental Materials. 2017 Feb 1;33(2):e62-e68. https://doi.org/10.1016/j.dental.2016.09.036
Seseogullari-Dirihan, Roda ; Mutluay, Mustafa Murat ; Pashley, David Henry ; Tezvergil-Mutluay, Arzu. / Is the inactivation of dentin proteases by crosslinkers reversible?. In: Dental Materials. 2017 ; Vol. 33, No. 2. pp. e62-e68.
@article{7118f7ac8ca6460ca82313f5c6d53c21,
title = "Is the inactivation of dentin proteases by crosslinkers reversible?",
abstract = "Objective Inactivation of dentin proteases by crosslinkers has been suggested as a way to prevent the degradation of dentin collagen in the hybrid layer. However, it is not known if the inhibition is reversible. The aim of this study was to evaluate the inactivation effect of various crosslinkers on dentin protease activity over a period of 6 months. Methods Demineralized dentin beams (1 × 2 × 6 mm, n = 10/group) were treated with (1) 1{\%} glutaraldehyde (GA1), (2) 5{\%} glutaraldehyde (GA5), (3) 1{\%} grape seed extract (GS1), (4) 5{\%} grape seed extract (GS5), (5) 10{\%} sumac berry extract (S), (6) 20 μM curcumin (CR20), and (7) 200 μM curcumin (CR200) for 5 min. Untreated beams served as control. The beams were incubated up to 6 months and incubation media were used to analyze solubilized telopeptide (ICTP and CTX) fragments as indicators of MMP- and cathepsin K-mediated degradation after 1, 3 and 6 months of incubation. The relative MMP activity of dentin beams was tested using a generic MMP assay. Data were analyzed using repeated-measures ANOVA, α = 0.05. Results All treated groups showed significant decrease in CTX release (32.2–469.5 pg/mg dentin) and ICTP (1.8–47.6 ng/mg dentin) fragments during the first month of incubation compared to control (1159 pg/mg and 72.9 ng/mg dentin, respectively). GA5, GS5 and CR200 maintained their inhibitory effect during 6-month incubation. The results were confirmed by dry mass loss and relative MMP activity following 6 months. Significance The results of this study indicate that the long-term effect is both crosslinker and dose dependent.",
keywords = "Crosslinkers, Cysteine cathepsins, Degradation, Dentin, Matrix metalloproteinases",
author = "Roda Seseogullari-Dirihan and Mutluay, {Mustafa Murat} and Pashley, {David Henry} and Arzu Tezvergil-Mutluay",
year = "2017",
month = "2",
day = "1",
doi = "10.1016/j.dental.2016.09.036",
language = "English (US)",
volume = "33",
pages = "e62--e68",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "2",

}

TY - JOUR

T1 - Is the inactivation of dentin proteases by crosslinkers reversible?

AU - Seseogullari-Dirihan, Roda

AU - Mutluay, Mustafa Murat

AU - Pashley, David Henry

AU - Tezvergil-Mutluay, Arzu

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Objective Inactivation of dentin proteases by crosslinkers has been suggested as a way to prevent the degradation of dentin collagen in the hybrid layer. However, it is not known if the inhibition is reversible. The aim of this study was to evaluate the inactivation effect of various crosslinkers on dentin protease activity over a period of 6 months. Methods Demineralized dentin beams (1 × 2 × 6 mm, n = 10/group) were treated with (1) 1% glutaraldehyde (GA1), (2) 5% glutaraldehyde (GA5), (3) 1% grape seed extract (GS1), (4) 5% grape seed extract (GS5), (5) 10% sumac berry extract (S), (6) 20 μM curcumin (CR20), and (7) 200 μM curcumin (CR200) for 5 min. Untreated beams served as control. The beams were incubated up to 6 months and incubation media were used to analyze solubilized telopeptide (ICTP and CTX) fragments as indicators of MMP- and cathepsin K-mediated degradation after 1, 3 and 6 months of incubation. The relative MMP activity of dentin beams was tested using a generic MMP assay. Data were analyzed using repeated-measures ANOVA, α = 0.05. Results All treated groups showed significant decrease in CTX release (32.2–469.5 pg/mg dentin) and ICTP (1.8–47.6 ng/mg dentin) fragments during the first month of incubation compared to control (1159 pg/mg and 72.9 ng/mg dentin, respectively). GA5, GS5 and CR200 maintained their inhibitory effect during 6-month incubation. The results were confirmed by dry mass loss and relative MMP activity following 6 months. Significance The results of this study indicate that the long-term effect is both crosslinker and dose dependent.

AB - Objective Inactivation of dentin proteases by crosslinkers has been suggested as a way to prevent the degradation of dentin collagen in the hybrid layer. However, it is not known if the inhibition is reversible. The aim of this study was to evaluate the inactivation effect of various crosslinkers on dentin protease activity over a period of 6 months. Methods Demineralized dentin beams (1 × 2 × 6 mm, n = 10/group) were treated with (1) 1% glutaraldehyde (GA1), (2) 5% glutaraldehyde (GA5), (3) 1% grape seed extract (GS1), (4) 5% grape seed extract (GS5), (5) 10% sumac berry extract (S), (6) 20 μM curcumin (CR20), and (7) 200 μM curcumin (CR200) for 5 min. Untreated beams served as control. The beams were incubated up to 6 months and incubation media were used to analyze solubilized telopeptide (ICTP and CTX) fragments as indicators of MMP- and cathepsin K-mediated degradation after 1, 3 and 6 months of incubation. The relative MMP activity of dentin beams was tested using a generic MMP assay. Data were analyzed using repeated-measures ANOVA, α = 0.05. Results All treated groups showed significant decrease in CTX release (32.2–469.5 pg/mg dentin) and ICTP (1.8–47.6 ng/mg dentin) fragments during the first month of incubation compared to control (1159 pg/mg and 72.9 ng/mg dentin, respectively). GA5, GS5 and CR200 maintained their inhibitory effect during 6-month incubation. The results were confirmed by dry mass loss and relative MMP activity following 6 months. Significance The results of this study indicate that the long-term effect is both crosslinker and dose dependent.

KW - Crosslinkers

KW - Cysteine cathepsins

KW - Degradation

KW - Dentin

KW - Matrix metalloproteinases

UR - http://www.scopus.com/inward/record.url?scp=84994404276&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994404276&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2016.09.036

DO - 10.1016/j.dental.2016.09.036

M3 - Article

VL - 33

SP - e62-e68

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 2

ER -