Ischaemia-induced changes in canine cardiac sarcoplasmic reticulum

Kamon Imai, Taitzer Wang, Ronald W. Millard, Muhammad Ashraf, Evangelia G. Kranias, Goro Asano, Angela O.Grassi De Gende, Taku Nagao, R. John Solaro, Arnold Schwartz

Research output: Contribution to journalReview articlepeer-review

20 Scopus citations


Summary: Effects of myocardial ischaemia on sarcoplasmic reticulum (SR) of dog hearts were investigated. Regional ischaemia was produced by occlusion of the left circumflex artery, and a microsomal fraction enriched in vesicles of SR was isolated from subendocardium (Endo) and subepicardium (Epi) of control and ischaemic areas of the heart. No significant changes occurred in ischaemic Epi. A loss of in vitro activities (ie calcium transport and ATPase) was found for SR from ischaemic Endo which paralleled the changes in the histology of the tissue. At 5 min of coronary occlusion, Ca2+ binding and Ca2+-ATPase activities of SR from ischaemic Endo were normal. A decrease in the activities of SR was first evident at 15 min after the occlusion, decreased further at about 30 min and remained at that level at 60 min of ischaemia. The maximal rate of Ca2+ uptake did not parallel the Ca2+-binding and Ca2+-ATPase activities. The degree of cAMP-dependent phosphorylation by endogenous and exogenous protein kinase was not different between SR from control and ischaemic areas. A participatory role of SR in the ischaemic impairment of left ventricular systolic and diastolic performance is discussed.

Original languageEnglish (US)
Pages (from-to)696-709
Number of pages14
JournalCardiovascular Research
Issue number11
StatePublished - Jan 1 1983


  • Calcium transport
  • Dog heart
  • Myocardial ischaemia
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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