TY - JOUR
T1 - Isolated Bovine Spinal Motoneurons Have Specific Ganglioside Antigens Recognized by Sera from Patients with Motor Neuron Disease and Motor Neuropathy
AU - Yoshino, Hiide
AU - Miyatani, Nobuyuki
AU - Saito, Megumi
AU - Ariga, Toshio
AU - Lugaresi, Alessandra
AU - Latov, Norman
AU - Kushi, Yasunori
AU - Kasama, Takeshi
AU - Yu, Robert K.
PY - 1992/11
Y1 - 1992/11
N2 - Abstract: The gangliosides GM1 and GD1b have recently been reported to be potential target antigens in human motor neuron disease (MND) or motor neuropathy. The mechanism for selective motoneuron and motor nerve impairment by the antibodies directed against these gangliosides, however, is not fully understood. We recently investigated the ganglioside composition of isolated bovine spinal motoneurons and found that the ganglioside pattern of the isolated motoneurons was extremely complex. GM1, GD1a, GD1b, and GT1b, which are major ganglioside components of CNS tissues, were only minor species in motoneurons. Among the various ganglioside species in motoneurons, several were immunoreactive to sera from patients with MND and motor neuropathy. One of these gangliosides was purified from bovine spinal cord and characterized as N‐glycolylneuraminic acid‐containing GM1 [GM1(NeuGc)] by compositional analysis, fast atom bombardment mass spectra, and the use of specific antibodies. Among seven sera with anti‐GM1 antibody activities, five sera reacted with GM1(NeuGc) and two did not. Two other gangliosides, which were recognized by another patient's serum, appeared to be specific for motoneurons. We conclude that motoneurons contained, in addition to the known ganglioside antigens GM1 and GDlb, other specific ganglioside antigens that could be recognized by sera from patients with MND and motor neuropathy.
AB - Abstract: The gangliosides GM1 and GD1b have recently been reported to be potential target antigens in human motor neuron disease (MND) or motor neuropathy. The mechanism for selective motoneuron and motor nerve impairment by the antibodies directed against these gangliosides, however, is not fully understood. We recently investigated the ganglioside composition of isolated bovine spinal motoneurons and found that the ganglioside pattern of the isolated motoneurons was extremely complex. GM1, GD1a, GD1b, and GT1b, which are major ganglioside components of CNS tissues, were only minor species in motoneurons. Among the various ganglioside species in motoneurons, several were immunoreactive to sera from patients with MND and motor neuropathy. One of these gangliosides was purified from bovine spinal cord and characterized as N‐glycolylneuraminic acid‐containing GM1 [GM1(NeuGc)] by compositional analysis, fast atom bombardment mass spectra, and the use of specific antibodies. Among seven sera with anti‐GM1 antibody activities, five sera reacted with GM1(NeuGc) and two did not. Two other gangliosides, which were recognized by another patient's serum, appeared to be specific for motoneurons. We conclude that motoneurons contained, in addition to the known ganglioside antigens GM1 and GDlb, other specific ganglioside antigens that could be recognized by sera from patients with MND and motor neuropathy.
KW - GM1
KW - Ganglioside
KW - Motoneuron
KW - Motor neuron disease
KW - Motor neuropathy
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U2 - 10.1111/j.1471-4159.1992.tb10999.x
DO - 10.1111/j.1471-4159.1992.tb10999.x
M3 - Article
C2 - 1383424
AN - SCOPUS:0026778734
SN - 0022-3042
VL - 59
SP - 1684
EP - 1691
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -