TY - JOUR
T1 - Isolation and amino acid composition of the isotypes of a rat clara cell specific protein
AU - Singh, Gurmukh
AU - Singal, Savita
AU - Katyal, Sikandar L.
AU - Brown, William E.
AU - Gottron, Shirley A.
N1 - Funding Information:
This investigation was supported by Public Health Service grants CA 33717 and HL 28193 and by the Veterans Administration. We are grateful to Mari-Lou Wong-Chong for technical assistance, Linda Shab and Pat Wohlfarth for photography, and Pat Turkovich and Pam Trbovich for typing the manuscript.
PY - 1987
Y1 - 1987
N2 - A protein of molecular weight about 10,000 (Clara cell protein C) present in lung lavage fluid and specific to Clara cells has been shown to have three isotypes. The isotypes have been individually isolated and purified by a combination of molecular sieving, ion exchange chromatography, column chromatofocusing, and reverse phase chromatogra-phy. The protein was monitored by immunoblotting and its purity tested by silver staining of the gel following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. About 33.7% of the 10-kDa protein present in starting rat lavage was recovered in final pure preparation. The 10-kDa Clara cell protein amounted to 0.38% of the total protein in rat lung lavage fluid. The three isotypes were present in a ratio of 1.0:2.8:8.8, the amounts increasing with decreasing pI None of the isotypes bound to concanavalin A. The amino acid compositions of the three isotypes were similar and were remarkable in that the contents of leucine, glutamic acid, aspartic acid, serine, and proline were high, tyrosine was present in low amounts, and methionine and histidine were absent.
AB - A protein of molecular weight about 10,000 (Clara cell protein C) present in lung lavage fluid and specific to Clara cells has been shown to have three isotypes. The isotypes have been individually isolated and purified by a combination of molecular sieving, ion exchange chromatography, column chromatofocusing, and reverse phase chromatogra-phy. The protein was monitored by immunoblotting and its purity tested by silver staining of the gel following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. About 33.7% of the 10-kDa protein present in starting rat lavage was recovered in final pure preparation. The 10-kDa Clara cell protein amounted to 0.38% of the total protein in rat lung lavage fluid. The three isotypes were present in a ratio of 1.0:2.8:8.8, the amounts increasing with decreasing pI None of the isotypes bound to concanavalin A. The amino acid compositions of the three isotypes were similar and were remarkable in that the contents of leucine, glutamic acid, aspartic acid, serine, and proline were high, tyrosine was present in low amounts, and methionine and histidine were absent.
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U2 - 10.3109/01902148709069595
DO - 10.3109/01902148709069595
M3 - Article
C2 - 3319535
AN - SCOPUS:0023514086
SN - 0190-2148
VL - 13
SP - 299
EP - 309
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 3
ER -