Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

Toshio Ariga, Michiko Sekine, Robert K. Yu, Tadashi Miyatake

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A novel GDlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as GDlb (NeuGc)2 or II3 (NeuGc)2-GgOse4Cer.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume232
Issue number1
DOIs
StatePublished - Jul 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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