Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

Toshio Ariga, Michiko Sekine, Robert K Yu, Tadashi Miyatake

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A novel G Dlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as G Dlb (NeuGc) 2 or II 3 (NeuGc) 2 -GgOse 4 Cer.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume232
Issue number1
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

Fingerprint

Adrenal Medulla
Gangliosides
Column chromatography
Neuraminidase
N-Acetylneuraminic Acid
Sugars
Chromatography
Digestion
Tissue
Lipids
sialogangliosides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla. / Ariga, Toshio; Sekine, Michiko; Yu, Robert K; Miyatake, Tadashi.

In: Archives of Biochemistry and Biophysics, Vol. 232, No. 1, 01.01.1984, p. 305-309.

Research output: Contribution to journalArticle

Ariga, Toshio ; Sekine, Michiko ; Yu, Robert K ; Miyatake, Tadashi. / Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla. In: Archives of Biochemistry and Biophysics. 1984 ; Vol. 232, No. 1. pp. 305-309.
@article{0667ccaf87c44d3facb74dd0bae19f4a,
title = "Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla",
abstract = "A novel G Dlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7{\%} of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as G Dlb (NeuGc) 2 or II 3 (NeuGc) 2 -GgOse 4 Cer.",
author = "Toshio Ariga and Michiko Sekine and Yu, {Robert K} and Tadashi Miyatake",
year = "1984",
month = "1",
day = "1",
doi = "10.1016/0003-9861(84)90546-0",
language = "English (US)",
volume = "232",
pages = "305--309",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Isolation and characterization of a novel disialoganglioside from bovine adrenal medulla

AU - Ariga, Toshio

AU - Sekine, Michiko

AU - Yu, Robert K

AU - Miyatake, Tadashi

PY - 1984/1/1

Y1 - 1984/1/1

N2 - A novel G Dlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as G Dlb (NeuGc) 2 or II 3 (NeuGc) 2 -GgOse 4 Cer.

AB - A novel G Dlb ganglioside which contains only N-glycolylneuraminic acid was isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The concentration of this ganglioside was 0.9 nmol lipid-bound sialic acid per gram fresh tissue, which accounted for 2.7% of the disialoganglioside fraction. The structure was elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structure of this ganglioside was identified as G Dlb (NeuGc) 2 or II 3 (NeuGc) 2 -GgOse 4 Cer.

UR - http://www.scopus.com/inward/record.url?scp=0021202602&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021202602&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(84)90546-0

DO - 10.1016/0003-9861(84)90546-0

M3 - Article

C2 - 6547584

AN - SCOPUS:0021202602

VL - 232

SP - 305

EP - 309

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -