Isolation and characterization of a population of stem-like progenitor cells from an atypical meningioma

Prakash Rath, Douglas C. Miller, N. Scott Litofsky, Douglas C. Anthony, Qi Feng, Craig Franklin, Lirong Pei, Alan Free, Jimei Liu, Mingqiang Ren, Mark D. Kirk, Huidong Shi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The majority of meningiomas are benign tumors associated with favorable outcomes; however, the less common aggressive variants with unfavorable outcomes often recur and may be due to subpopulations of less-differentiated cells residing within the tumor. These subpopulations of tumor cells have tumor-initiating properties and may be isolated from heterogeneous tumors when sorted or cultured in defined medium. We report the isolation and characterization of a population of tumor-initiating cells derived from an atypical meningioma. We identify a tumor-initiating population from an atypical meningioma, termed meningioma-initiating cells (MICs). These MICs self-renew, differentiate, and can recapitulate the histological characteristics of the parental tumor when transplanted at 1000 cells into the flank regions of athymic nude mice. Immunohistochemistry reveals stem-like protein expression patterns similar to neural stem and progenitor cells (NSPCs) while genomic profiling verified the isolation of cancer cells (with defined meningioma chromosomal aberrations) from the bulk tumor. Microarray and pathway analysis identifies biochemical processes and gene networks related to aberrant cell cycle progression, particularly the loss of heterozygosity of tumor suppressor genes CDKN2A (p16INK4A), p14ARF, and CDKN2B (p15INK4B). Flow cytometric analysis revealed the expression of CD44 and activated leukocyte adhesion molecule (ALCAM/CD166); these may prove to be markers able to identify this cell type. The isolation and identification of a tumor-initiating cell population capable of forming meningiomas demonstrates a useful model for understanding meningioma development. This meningioma model may be used to study the cell hierarchy of meningioma tumorogenesis and provide increased understanding of malignant progression.

Original languageEnglish (US)
Pages (from-to)179-188
Number of pages10
JournalExperimental and Molecular Pathology
Volume90
Issue number2
DOIs
StatePublished - Apr 1 2011

Fingerprint

Meningioma
Tumors
Stem Cells
Population
Cells
Neoplastic Stem Cells
Neoplasms
Nude Mice
Activated-Leukocyte Cell Adhesion Molecule
Biochemical Phenomena
Tumor Suppressor Protein p14ARF
Genes
Neural Stem Cells
Cell Separation
Gene Regulatory Networks
Loss of Heterozygosity
Cell Adhesion Molecules
Microarray Analysis
Tumor Suppressor Genes
Chromosome Aberrations

Keywords

  • Atypical meningioma
  • Malignant progression
  • Microarray
  • Molecular genetics
  • Progenitor cell

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Isolation and characterization of a population of stem-like progenitor cells from an atypical meningioma. / Rath, Prakash; Miller, Douglas C.; Litofsky, N. Scott; Anthony, Douglas C.; Feng, Qi; Franklin, Craig; Pei, Lirong; Free, Alan; Liu, Jimei; Ren, Mingqiang; Kirk, Mark D.; Shi, Huidong.

In: Experimental and Molecular Pathology, Vol. 90, No. 2, 01.04.2011, p. 179-188.

Research output: Contribution to journalArticle

Rath, P, Miller, DC, Litofsky, NS, Anthony, DC, Feng, Q, Franklin, C, Pei, L, Free, A, Liu, J, Ren, M, Kirk, MD & Shi, H 2011, 'Isolation and characterization of a population of stem-like progenitor cells from an atypical meningioma', Experimental and Molecular Pathology, vol. 90, no. 2, pp. 179-188. https://doi.org/10.1016/j.yexmp.2010.12.003
Rath, Prakash ; Miller, Douglas C. ; Litofsky, N. Scott ; Anthony, Douglas C. ; Feng, Qi ; Franklin, Craig ; Pei, Lirong ; Free, Alan ; Liu, Jimei ; Ren, Mingqiang ; Kirk, Mark D. ; Shi, Huidong. / Isolation and characterization of a population of stem-like progenitor cells from an atypical meningioma. In: Experimental and Molecular Pathology. 2011 ; Vol. 90, No. 2. pp. 179-188.
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