TY - JOUR
T1 - Isolation and characterization of a species-specific DNA probe for Candida albicans
AU - Oren, Iiana
AU - Manavathu, Elias K.
AU - Lerner, Stephen A.
PY - 1991/12/25
Y1 - 1991/12/25
N2 - As a model for the isolation of species-specific sequences of ONA, we Isolated and characterized a species-specific DNA fragment from the Candida albicans genome. We used a series of differential colony hybridization experiments, in which a C. albicans genomic library was hybridized with genomic DNA probes from related organisms to minimize the number of potentially specific C. albicans DMA fragments to bs tested. Sin clones were tested by dot blot analysis, and one of them, a 1348 bp EcoRI ONA fragment, was confirmed as specific for C. albicans. his species-specific fragment could be utilized as a DNA probe for rapid, sensitive, and specific diagnosis of C. albicans DNA in clinical specimens.
AB - As a model for the isolation of species-specific sequences of ONA, we Isolated and characterized a species-specific DNA fragment from the Candida albicans genome. We used a series of differential colony hybridization experiments, in which a C. albicans genomic library was hybridized with genomic DNA probes from related organisms to minimize the number of potentially specific C. albicans DMA fragments to bs tested. Sin clones were tested by dot blot analysis, and one of them, a 1348 bp EcoRI ONA fragment, was confirmed as specific for C. albicans. his species-specific fragment could be utilized as a DNA probe for rapid, sensitive, and specific diagnosis of C. albicans DNA in clinical specimens.
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U2 - 10.1093/nar/19.25.7113
DO - 10.1093/nar/19.25.7113
M3 - Article
C2 - 1766872
AN - SCOPUS:0026318818
SN - 0305-1048
VL - 19
SP - 7113
EP - 7116
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 25
ER -