Isolation and characterization of an apoptotic suppressor gene from spodoptera littoralis nucleopolyhedrovirus

Quansheng Du, Yipeng Qi, Chejanovsky Nor

Research output: Contribution to journalArticle

Abstract

The Spodoptera littoralis nucleopolyhedrovirus was able to suppress apoptosis triggered by an AcNPV p35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome, were individually cotransfected along with genomic DNA of an AcNPV p35 null mutant. Using this complementation assay we identified a novel SlNPV gene, inhibitor of apoptosis, SI p49. The Sl p49 sequence predicted a 49-KD polypeptide with about 48.8% identity to p35 of AcNPV. Recombinant AcNPVs, deficient in p35 and bearing the SI p49 gene, showed successful productive infection in Sf9 cells indicating that SI p49 is a functional homolog of p35.

Original languageEnglish (US)
JournalWuhan Daxue Xuebao/Journal of Wuhan University
Volume44
Issue number4
StatePublished - Dec 1 1998

Fingerprint

Genes
Cell death
Bearings (structural)
Polypeptides
Assays
DNA
Nucleopolyhedrovirus
Isolation
Gene
Apoptosis
Overlapping
Infection
Inhibitor
Recombinant

Keywords

  • Apoptosis
  • Nucleoplyhedrovirus
  • Slp49

ASJC Scopus subject areas

  • Management of Technology and Innovation

Cite this

Isolation and characterization of an apoptotic suppressor gene from spodoptera littoralis nucleopolyhedrovirus. / Du, Quansheng; Qi, Yipeng; Nor, Chejanovsky.

In: Wuhan Daxue Xuebao/Journal of Wuhan University, Vol. 44, No. 4, 01.12.1998.

Research output: Contribution to journalArticle

@article{2e46e4995622497b8d5b25d59f196ea5,
title = "Isolation and characterization of an apoptotic suppressor gene from spodoptera littoralis nucleopolyhedrovirus",
abstract = "The Spodoptera littoralis nucleopolyhedrovirus was able to suppress apoptosis triggered by an AcNPV p35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome, were individually cotransfected along with genomic DNA of an AcNPV p35 null mutant. Using this complementation assay we identified a novel SlNPV gene, inhibitor of apoptosis, SI p49. The Sl p49 sequence predicted a 49-KD polypeptide with about 48.8{\%} identity to p35 of AcNPV. Recombinant AcNPVs, deficient in p35 and bearing the SI p49 gene, showed successful productive infection in Sf9 cells indicating that SI p49 is a functional homolog of p35.",
keywords = "Apoptosis, Nucleoplyhedrovirus, Slp49",
author = "Quansheng Du and Yipeng Qi and Chejanovsky Nor",
year = "1998",
month = "12",
day = "1",
language = "English (US)",
volume = "44",
journal = "Wuhan Daxue Xuebao/Journal of Wuhan University",
issn = "0253-9888",
number = "4",

}

TY - JOUR

T1 - Isolation and characterization of an apoptotic suppressor gene from spodoptera littoralis nucleopolyhedrovirus

AU - Du, Quansheng

AU - Qi, Yipeng

AU - Nor, Chejanovsky

PY - 1998/12/1

Y1 - 1998/12/1

N2 - The Spodoptera littoralis nucleopolyhedrovirus was able to suppress apoptosis triggered by an AcNPV p35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome, were individually cotransfected along with genomic DNA of an AcNPV p35 null mutant. Using this complementation assay we identified a novel SlNPV gene, inhibitor of apoptosis, SI p49. The Sl p49 sequence predicted a 49-KD polypeptide with about 48.8% identity to p35 of AcNPV. Recombinant AcNPVs, deficient in p35 and bearing the SI p49 gene, showed successful productive infection in Sf9 cells indicating that SI p49 is a functional homolog of p35.

AB - The Spodoptera littoralis nucleopolyhedrovirus was able to suppress apoptosis triggered by an AcNPV p35 null mutant. To identify the putative apoptotic suppressor gene of SlNPV, overlapping cosmid clones representing the entire SlNPV genome, were individually cotransfected along with genomic DNA of an AcNPV p35 null mutant. Using this complementation assay we identified a novel SlNPV gene, inhibitor of apoptosis, SI p49. The Sl p49 sequence predicted a 49-KD polypeptide with about 48.8% identity to p35 of AcNPV. Recombinant AcNPVs, deficient in p35 and bearing the SI p49 gene, showed successful productive infection in Sf9 cells indicating that SI p49 is a functional homolog of p35.

KW - Apoptosis

KW - Nucleoplyhedrovirus

KW - Slp49

UR - http://www.scopus.com/inward/record.url?scp=11544357716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11544357716&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:11544357716

VL - 44

JO - Wuhan Daxue Xuebao/Journal of Wuhan University

JF - Wuhan Daxue Xuebao/Journal of Wuhan University

SN - 0253-9888

IS - 4

ER -