Isolation and characterization of ganglioside G(M1b) from normal human brain

T. Ariga; R. K. Yu

Isolation and characterization of ganglioside G(M1b) from normal human brain

T. Ariga, R. K. Yu

Research output: Contribution to journal › Article › peer-review

Abstract

A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV³NeuAcGgOse₄Cer.

Original language	English (US)
Pages (from-to)	285-291
Number of pages	7
Journal	Journal of Lipid Research
Volume	28
Issue number	3
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Endocrinology
Cell Biology

Cite this

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title = "Isolation and characterization of ganglioside G(M1b) from normal human brain",

abstract = "A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV3NeuAcGgOse4Cer.",

author = "T. Ariga and Yu, {R. K.}",

year = "1987",

language = "English (US)",

volume = "28",

pages = "285--291",

journal = "Journal of Lipid Research",

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T1 - Isolation and characterization of ganglioside G(M1b) from normal human brain

AU - Ariga, T.

AU - Yu, R. K.

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N2 - A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV3NeuAcGgOse4Cer.

AB - A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV3NeuAcGgOse4Cer.

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Isolation and characterization of ganglioside G(M1b) from normal human brain

Abstract

ASJC Scopus subject areas

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