Isolation and characterization of ganglioside G(M1b) from normal human brain

T. Ariga, R. K. Yu

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV3NeuAcGgOse4Cer.

Original languageEnglish (US)
Pages (from-to)285-291
Number of pages7
JournalJournal of Lipid Research
Volume28
Issue number3
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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