A sialidase-susceptible monosialoganglioside was isolated from normal human brain by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The yield of this ganglioside was about 6 mg per whole brain. Its structure was elucidated by sugar analysis, sialidase digestion, permethylation, and proton NMR studies. This ganglioside had carbohydrate, fatty acid, and long-chain base compositions identical to those of brain G(M1a). However, the sialosyl residue was found to be linked (α2-3) to the terminal galactosyl residue of the asialo-G(M1a) backbone. The complete structure of this ganglioside was therefore identified as G(M1b) or IV3NeuAcGgOse4Cer.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Lipid Research|
|State||Published - 1987|
ASJC Scopus subject areas
- Cell Biology