Isolation and characterization of riboflavin carrier protein from human amniotic fluid

Puttur D Prasad, P. Malhotra, A. A. Karande, P. R. Adiga

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A specific protein exhibiting immunological cross-reactivity with chicken riboflavin carrier protein has been purified to homogeneity from human amniotic fluid by use of ion-exchange and affinity chromatography. The protein is similar to its avian counterpart in terms of molecular size, distribution of 125I-labelled tryptic peptides during finger printing, and preferential binding to riboflavin. Immunologically, they are homologous since most of the monoclonal antibodies raised against the avian protein cross-react with the purified human vitamin carrier.

Original languageEnglish (US)
Pages (from-to)385-395
Number of pages11
JournalBiochemistry International
Volume27
Issue number3
StatePublished - Jan 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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