Isolation and characterization of the trisialogangliosides from bovine adrenal medulla.

T. Ariga, M. Sekine, Robert K Yu, T. Miyatake

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Trisialogangliosides were isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. Their structures were elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structures of trisialogangliosides, A to D, were identified as follows. A: GT1b, IV3NeuAc, II3 (NeuAc)2-GgOse4Cer. B: GT1b(NeuAc/NeuAc-NeuGc-); IV3NeuAc, II3 (NeuAc alpha 2-8 NeuGc-)GgOse4Cer. C: GT1b (NeuGc/NeuAc-NeuAc-); IV3NeuGc, II3 (NeuAc alpha 2-8 NeuAc-)GgOse4Cer. D: GT1b (NeuAc/NeuGc-NeuGc-); IV3NeuAc, II3 (NeuGc alpha 2-8 NeuGc-)GgOse4Cer. Gangliosides B, C, and D, which contain N-glycolylneuraminic acid, have not previously been reported in the literature.

Original languageEnglish (US)
Pages (from-to)737-745
Number of pages9
JournalJournal of Lipid Research
Volume24
Issue number6
StatePublished - Jun 1 1983
Externally publishedYes

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Adrenal Medulla
Column chromatography
Gangliosides
Neuraminidase
Sugars
Chromatography
Digestion
trisialoganglioside
DEAE-sephadex A-25
N-glycolylneuraminic acid
NeuAc-2-8-NeuAc

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Isolation and characterization of the trisialogangliosides from bovine adrenal medulla. / Ariga, T.; Sekine, M.; Yu, Robert K; Miyatake, T.

In: Journal of Lipid Research, Vol. 24, No. 6, 01.06.1983, p. 737-745.

Research output: Contribution to journalArticle

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