Isolation and characterization of the trisialogangliosides from bovine adrenal medulla.

T. Ariga, M. Sekine, R. K. Yu, T. Miyatake

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Trisialogangliosides were isolated from bovine adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. Their structures were elucidated by sugar analysis, neuraminidase digestion, and permethylation studies. The complete structures of trisialogangliosides, A to D, were identified as follows. A: GT1b, IV3NeuAc, II3 (NeuAc)2-GgOse4Cer. B: GT1b(NeuAc/NeuAc-NeuGc-); IV3NeuAc, II3 (NeuAc alpha 2-8 NeuGc-)GgOse4Cer. C: GT1b (NeuGc/NeuAc-NeuAc-); IV3NeuGc, II3 (NeuAc alpha 2-8 NeuAc-)GgOse4Cer. D: GT1b (NeuAc/NeuGc-NeuGc-); IV3NeuAc, II3 (NeuGc alpha 2-8 NeuGc-)GgOse4Cer. Gangliosides B, C, and D, which contain N-glycolylneuraminic acid, have not previously been reported in the literature.

Original languageEnglish (US)
Pages (from-to)737-745
Number of pages9
JournalJournal of Lipid Research
Issue number6
Publication statusPublished - Jun 1 1983
Externally publishedYes


ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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