TY - JOUR
T1 - Isolation and culture of retinal microglia
AU - Roque, R. S.
AU - Caldwell, R. B.
N1 - Funding Information:
ACKNOWLEDGEMENTS This work was done during the tenure of a research fellowship from the American Heart Association, Georgia Miliate. It was also supported by BRSG #S-07-RR5635-30 and NIH EY-04618. MCSF was obtained from Genetics Institute, Boston, MA, while the monoclonal antibody against Mac-la was
PY - 1993
Y1 - 1993
N2 - In the Royal College of Surgeons rat, the migration of phagocytic cells into the subretinal space accompanies photoreceptor cell death during the early stages of retinal dystrophy. These are followed closely by cellular alterations in the retinal pigment epithelium, Müller cells, and outer retinal vessels. We have identified the phagocytic cells as microglia and hypothesized that they may be involved in the above cellular changes. Thus, we developed procedures for their isolation and growth. Our study shows that retina-derived microglia (1) are positive for microglial markers Griffonia simplicifolia isolectin B4, Mac-1α phosphotyrosine, and vimentin; (2) are highly phagocytic; and (3) respond to macrophage colony stimulating factor by proliferating. This culture system would provide a valuable tool in studying mechanisms of cellular alterations in retinal disease.
AB - In the Royal College of Surgeons rat, the migration of phagocytic cells into the subretinal space accompanies photoreceptor cell death during the early stages of retinal dystrophy. These are followed closely by cellular alterations in the retinal pigment epithelium, Müller cells, and outer retinal vessels. We have identified the phagocytic cells as microglia and hypothesized that they may be involved in the above cellular changes. Thus, we developed procedures for their isolation and growth. Our study shows that retina-derived microglia (1) are positive for microglial markers Griffonia simplicifolia isolectin B4, Mac-1α phosphotyrosine, and vimentin; (2) are highly phagocytic; and (3) respond to macrophage colony stimulating factor by proliferating. This culture system would provide a valuable tool in studying mechanisms of cellular alterations in retinal disease.
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U2 - 10.3109/02713689308999475
DO - 10.3109/02713689308999475
M3 - Article
C2 - 7683260
AN - SCOPUS:0027245655
SN - 0271-3683
VL - 12
SP - 285
EP - 290
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -