Isolation and culture of retinal microglia

R. S. Roque, Ruth B Caldwell

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In the Royal College of Surgeons rat, the migration of phagocytic cells into the subretinal space accompanies photoreceptor cell death during the early stages of retinal dystrophy. These are followed closely by cellular alterations in the retinal pigment epithelium, Müller cells, and outer retinal vessels. We have identified the phagocytic cells as microglia and hypothesized that they may be involved in the above cellular changes. Thus, we developed procedures for their isolation and growth. Our study shows that retina-derived microglia (1) are positive for microglial markers Griffonia simplicifolia isolectin B4, Mac-1α phosphotyrosine, and vimentin; (2) are highly phagocytic; and (3) respond to macrophage colony stimulating factor by proliferating. This culture system would provide a valuable tool in studying mechanisms of cellular alterations in retinal disease.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalCurrent Eye Research
Volume12
Issue number3
DOIs
StatePublished - Jan 1 1993

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Microglia
Phagocytes
Retinal Dystrophies
Retinal Diseases
Retinal Vessels
Photoreceptor Cells
Phosphotyrosine
Macrophage Colony-Stimulating Factor
Retinal Pigment Epithelium
Vimentin
Retina
Cell Death
Growth
Griffonia simplicifolia lectins
Surgeons

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Isolation and culture of retinal microglia. / Roque, R. S.; Caldwell, Ruth B.

In: Current Eye Research, Vol. 12, No. 3, 01.01.1993, p. 285-290.

Research output: Contribution to journalArticle

Roque, R. S. ; Caldwell, Ruth B. / Isolation and culture of retinal microglia. In: Current Eye Research. 1993 ; Vol. 12, No. 3. pp. 285-290.
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