Isolation and functional analysis of the promoter of the rat CMP-NeuAc:GM3 α 2,8 sialyltransferase gene

Guichao Zeng, Luoyi Gao, Robert K Yu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A 2.1-kb 5′-flanking fragment of the rat CMP-NeuAc:GM3 α2,8 sialyltransferase (GD3-synthase) gene was cloned by the genomic walking procedure. The promoter activity of the fragment was assessed in F-11 cells by transient transfection and the locations for the basal and maximal promoter activities were defined. Primer extension analysis identified a transcription start site approximately 98 bp upstream of the ATG start codon. DNA sequence analysis of the promoter revealed a number of consensus binding sites for known transcription factors such as SP1, AP1, NFk-B, C/EBP and TFIID, and a repeat GC-GT sequence motif seen for the formation of Z-type DNA. Both TATA and CCAAT boxes were not found in the promoter. Our results from deletion constructs suggested that both positive and negative cis-acting regulatory regions were present in this TATA-less promoter of the rat GD3-synthase gene.

Original languageEnglish (US)
Pages (from-to)126-130
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1397
Issue number2
StatePublished - Apr 27 1998
Externally publishedYes

Fingerprint

Sialyltransferases
Cytidine Monophosphate
Functional analysis
Rats
Genes
Transcription Factor TFIID
Z-Form DNA
TATA Box
Initiator Codon
Nucleic Acid Regulatory Sequences
Transcription Initiation Site
DNA sequences
DNA Sequence Analysis
Walking
Transfection
Transcription Factors
Binding Sites

Keywords

  • GD3-synthase
  • Ganglioside
  • Gene expression
  • Sialyltransferase
  • Transcription

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Isolation and functional analysis of the promoter of the rat CMP-NeuAc:GM3 α 2,8 sialyltransferase gene. / Zeng, Guichao; Gao, Luoyi; Yu, Robert K.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1397, No. 2, 27.04.1998, p. 126-130.

Research output: Contribution to journalArticle

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