Isolation and functional analysis of the promoter of the rat CMP-NeuAc:GM3 α 2,8 sialyltransferase gene

Guichao Zeng, Luoyi Gao, Robert K. Yu

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A 2.1-kb 5′-flanking fragment of the rat CMP-NeuAc:GM3 α2,8 sialyltransferase (GD3-synthase) gene was cloned by the genomic walking procedure. The promoter activity of the fragment was assessed in F-11 cells by transient transfection and the locations for the basal and maximal promoter activities were defined. Primer extension analysis identified a transcription start site approximately 98 bp upstream of the ATG start codon. DNA sequence analysis of the promoter revealed a number of consensus binding sites for known transcription factors such as SP1, AP1, NFk-B, C/EBP and TFIID, and a repeat GC-GT sequence motif seen for the formation of Z-type DNA. Both TATA and CCAAT boxes were not found in the promoter. Our results from deletion constructs suggested that both positive and negative cis-acting regulatory regions were present in this TATA-less promoter of the rat GD3-synthase gene.

Original languageEnglish (US)
Pages (from-to)126-130
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1397
Issue number2
Publication statusPublished - Apr 27 1998
Externally publishedYes

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Keywords

  • GD3-synthase
  • Ganglioside
  • Gene expression
  • Sialyltransferase
  • Transcription

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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