Isolation and regional localisation of DNA sequences from a human chromosome 11-specific cosmid library

Roy B. Wadey, Peter F.R. Little, Jon Pritchard, John Kenneth Cowell

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A cosmid library has been prepared in the lorist-B vector from a mouse/human somatic cell hybrid containing region 11q23-11pter as the only human component. This chromosome region is stably maintained in the hybrid as a result of translocation onto one copy of mouse chromosome 13. Individual cosmids containing human DNA were isolated by their ability to hybridise with total human DNA, digested with either HindIII or EcoRI, and 33 individual unique sequences were identified. These fragments were then isolated and subcloned into the bluescribe plasmid vector. Regional localisation of these unique sequences was achieved using a panel of somatic cell hybrids containing different overlapping deletions of chromosome 11. The majority of the 33 mapped sequences derived from the long arm of chromosome 11. Two clones were located within the 11p13-p14 region, which is associated with a predisposition to Wilms' tumour. These probes supplement those already mapped to this chromosome and will assist in the generation of a detailed chromosome 11 linkage map.

Original languageEnglish (US)
Pages (from-to)417-423
Number of pages7
JournalHuman Genetics
Volume84
Issue number5
DOIs
StatePublished - Apr 1 1990
Externally publishedYes

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Cosmids
Chromosomes, Human, Pair 11
Human Chromosomes
Hybrid Cells
Chromosomes
Chromosomes, Human, Pair 13
Wilms Tumor
DNA
Plasmids
Clone Cells

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Isolation and regional localisation of DNA sequences from a human chromosome 11-specific cosmid library. / Wadey, Roy B.; Little, Peter F.R.; Pritchard, Jon; Cowell, John Kenneth.

In: Human Genetics, Vol. 84, No. 5, 01.04.1990, p. 417-423.

Research output: Contribution to journalArticle

Wadey, Roy B. ; Little, Peter F.R. ; Pritchard, Jon ; Cowell, John Kenneth. / Isolation and regional localisation of DNA sequences from a human chromosome 11-specific cosmid library. In: Human Genetics. 1990 ; Vol. 84, No. 5. pp. 417-423.
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