Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel

R. H.J. Houwen, S. E. Pautler, J. A. Barwell, K. Arden, J. A. Buchanan, C. D. James, W. K. Cavenee, C. H.C.M. Buys, John Kenneth Cowell, D. W. Cox

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Clones were isolated from two flow-sorted chromosome 13 libraries. Twenty-five clones were localized to various regions of chromosome 13, using a well-characterized panel of rodent x human hybrid cell lines. Eight DNA markers were localized to 13q14.2→q22, where the gene for Wilson disease, a recessive disorder of copper metabolism, was previously assigned. The new markers will be useful for the diagnosis of pre-symptomatic sibs of Wilson disease patients. We isolated six DNA clones proximal to the retinoblastoma gene, a region in which a translocation associated with rhabdomyosarcoma has been observed. Probes for both of these regions will be useful for the cloning of the genes involved in these diseases.

Original languageEnglish (US)
Pages (from-to)87-90
Number of pages4
JournalCytogenetic and Genome Research
Volume57
Issue number2-3
DOIs
StatePublished - Jan 1 1991

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Chromosomes, Human, Pair 13
Hepatolenticular Degeneration
Clone Cells
Retinoblastoma Genes
Rhabdomyosarcoma
Hybrid Cells
Genetic Markers
Genes
Organism Cloning
Copper
Rodentia
Cell Line
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Houwen, R. H. J., Pautler, S. E., Barwell, J. A., Arden, K., Buchanan, J. A., James, C. D., ... Cox, D. W. (1991). Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel. Cytogenetic and Genome Research, 57(2-3), 87-90. https://doi.org/10.1159/000133120

Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel. / Houwen, R. H.J.; Pautler, S. E.; Barwell, J. A.; Arden, K.; Buchanan, J. A.; James, C. D.; Cavenee, W. K.; Buys, C. H.C.M.; Cowell, John Kenneth; Cox, D. W.

In: Cytogenetic and Genome Research, Vol. 57, No. 2-3, 01.01.1991, p. 87-90.

Research output: Contribution to journalArticle

Houwen, RHJ, Pautler, SE, Barwell, JA, Arden, K, Buchanan, JA, James, CD, Cavenee, WK, Buys, CHCM, Cowell, JK & Cox, DW 1991, 'Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel', Cytogenetic and Genome Research, vol. 57, no. 2-3, pp. 87-90. https://doi.org/10.1159/000133120
Houwen, R. H.J. ; Pautler, S. E. ; Barwell, J. A. ; Arden, K. ; Buchanan, J. A. ; James, C. D. ; Cavenee, W. K. ; Buys, C. H.C.M. ; Cowell, John Kenneth ; Cox, D. W. / Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel. In: Cytogenetic and Genome Research. 1991 ; Vol. 57, No. 2-3. pp. 87-90.
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