Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel

R. H.J. Houwen, S. E. Pautler, J. A. Barwell, K. Arden, J. A. Buchanan, C. D. James, W. K. Cavenee, C. H.C.M. Buys, John Kenneth Cowell, D. W. Cox

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Clones were isolated from two flow-sorted chromosome 13 libraries. Twenty-five clones were localized to various regions of chromosome 13, using a well-characterized panel of rodent x human hybrid cell lines. Eight DNA markers were localized to 13q14.2→q22, where the gene for Wilson disease, a recessive disorder of copper metabolism, was previously assigned. The new markers will be useful for the diagnosis of pre-symptomatic sibs of Wilson disease patients. We isolated six DNA clones proximal to the retinoblastoma gene, a region in which a translocation associated with rhabdomyosarcoma has been observed. Probes for both of these regions will be useful for the cloning of the genes involved in these diseases.

Original languageEnglish (US)
Pages (from-to)87-90
Number of pages4
JournalCytogenetic and Genome Research
Volume57
Issue number2-3
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel'. Together they form a unique fingerprint.

  • Cite this

    Houwen, R. H. J., Pautler, S. E., Barwell, J. A., Arden, K., Buchanan, J. A., James, C. D., Cavenee, W. K., Buys, C. H. C. M., Cowell, J. K., & Cox, D. W. (1991). Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel. Cytogenetic and Genome Research, 57(2-3), 87-90. https://doi.org/10.1159/000133120