Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor

John A. Lust, Kathleen A. Donovan, Michael P. Kline, Philip R. Greipp, Robert A. Kyle, Nita Jane Maihle

Research output: Contribution to journalArticle

240 Citations (Scopus)

Abstract

Soluble forms of the interleukin (IL)-2, IL-4 and IL-7 receptors which lack the transmembrane domain have been described. IL-6 is a growth factor important in the final differentiation of B-cells into plasma cells and in the pathogenesis of multiple myeloma. To determine whether the receptor for IL-6 may exist as a soluble molecule, RNA was analysed from the transformed B-cell lines U266, CESS and Daudi, from bone marrow from two myeloma patients, and from normal leukocytes. Using polymerase chain reaction, oligonucleotide primers which flank the transmembrane domain were selected to generate a 339 bp fragment. All samples produced equivalent amounts of the expected 339 bp fragment plus a smaller 245 bp fragment except Daudi which exhibited virtual absence of both. Sequence analysis of the smaller fragments from each of the five samples demonstrated the deletion of the entire transmembrane region from codons 356 (G-TG) to 387 (AG-G). The boundaries of this deletion were identical in all cases. Partial sequence analysis of the ligand-binding domain for U266 demonstrated identical sequences for the membrane-bound and soluble forms of the IL-6 receptor cDNAs. In summary, an mRNA which encodes a soluble form of the IL-6 receptor is expressed in both normal and myeloma cells.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalCytokine
Volume4
Issue number2
DOIs
StatePublished - Jan 1 1992

Fingerprint

Interleukin-6 Receptors
Messenger RNA
Sequence Analysis
B-Lymphocytes
Interleukin-7 Receptors
Cells
Transformed Cell Line
DNA Primers
Polymerase chain reaction
Plasma Cells
Multiple Myeloma
Codon
Interleukin-4
Interleukin-2
Interleukin-6
Intercellular Signaling Peptides and Proteins
Bone
Leukocytes
Complementary DNA
Bone Marrow

Keywords

  • interleukin-6
  • myeloma
  • soluble receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Lust, J. A., Donovan, K. A., Kline, M. P., Greipp, P. R., Kyle, R. A., & Maihle, N. J. (1992). Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. Cytokine, 4(2), 96-100. https://doi.org/10.1016/1043-4666(92)90043-Q

Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. / Lust, John A.; Donovan, Kathleen A.; Kline, Michael P.; Greipp, Philip R.; Kyle, Robert A.; Maihle, Nita Jane.

In: Cytokine, Vol. 4, No. 2, 01.01.1992, p. 96-100.

Research output: Contribution to journalArticle

Lust, JA, Donovan, KA, Kline, MP, Greipp, PR, Kyle, RA & Maihle, NJ 1992, 'Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor', Cytokine, vol. 4, no. 2, pp. 96-100. https://doi.org/10.1016/1043-4666(92)90043-Q
Lust JA, Donovan KA, Kline MP, Greipp PR, Kyle RA, Maihle NJ. Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. Cytokine. 1992 Jan 1;4(2):96-100. https://doi.org/10.1016/1043-4666(92)90043-Q
Lust, John A. ; Donovan, Kathleen A. ; Kline, Michael P. ; Greipp, Philip R. ; Kyle, Robert A. ; Maihle, Nita Jane. / Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor. In: Cytokine. 1992 ; Vol. 4, No. 2. pp. 96-100.
@article{817a493efaf04d01b82f401f35e9f6fb,
title = "Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor",
abstract = "Soluble forms of the interleukin (IL)-2, IL-4 and IL-7 receptors which lack the transmembrane domain have been described. IL-6 is a growth factor important in the final differentiation of B-cells into plasma cells and in the pathogenesis of multiple myeloma. To determine whether the receptor for IL-6 may exist as a soluble molecule, RNA was analysed from the transformed B-cell lines U266, CESS and Daudi, from bone marrow from two myeloma patients, and from normal leukocytes. Using polymerase chain reaction, oligonucleotide primers which flank the transmembrane domain were selected to generate a 339 bp fragment. All samples produced equivalent amounts of the expected 339 bp fragment plus a smaller 245 bp fragment except Daudi which exhibited virtual absence of both. Sequence analysis of the smaller fragments from each of the five samples demonstrated the deletion of the entire transmembrane region from codons 356 (G-TG) to 387 (AG-G). The boundaries of this deletion were identical in all cases. Partial sequence analysis of the ligand-binding domain for U266 demonstrated identical sequences for the membrane-bound and soluble forms of the IL-6 receptor cDNAs. In summary, an mRNA which encodes a soluble form of the IL-6 receptor is expressed in both normal and myeloma cells.",
keywords = "interleukin-6, myeloma, soluble receptor",
author = "Lust, {John A.} and Donovan, {Kathleen A.} and Kline, {Michael P.} and Greipp, {Philip R.} and Kyle, {Robert A.} and Maihle, {Nita Jane}",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/1043-4666(92)90043-Q",
language = "English (US)",
volume = "4",
pages = "96--100",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Isolation of an mRNA encoding a soluble form of the human interleukin-6 receptor

AU - Lust, John A.

AU - Donovan, Kathleen A.

AU - Kline, Michael P.

AU - Greipp, Philip R.

AU - Kyle, Robert A.

AU - Maihle, Nita Jane

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Soluble forms of the interleukin (IL)-2, IL-4 and IL-7 receptors which lack the transmembrane domain have been described. IL-6 is a growth factor important in the final differentiation of B-cells into plasma cells and in the pathogenesis of multiple myeloma. To determine whether the receptor for IL-6 may exist as a soluble molecule, RNA was analysed from the transformed B-cell lines U266, CESS and Daudi, from bone marrow from two myeloma patients, and from normal leukocytes. Using polymerase chain reaction, oligonucleotide primers which flank the transmembrane domain were selected to generate a 339 bp fragment. All samples produced equivalent amounts of the expected 339 bp fragment plus a smaller 245 bp fragment except Daudi which exhibited virtual absence of both. Sequence analysis of the smaller fragments from each of the five samples demonstrated the deletion of the entire transmembrane region from codons 356 (G-TG) to 387 (AG-G). The boundaries of this deletion were identical in all cases. Partial sequence analysis of the ligand-binding domain for U266 demonstrated identical sequences for the membrane-bound and soluble forms of the IL-6 receptor cDNAs. In summary, an mRNA which encodes a soluble form of the IL-6 receptor is expressed in both normal and myeloma cells.

AB - Soluble forms of the interleukin (IL)-2, IL-4 and IL-7 receptors which lack the transmembrane domain have been described. IL-6 is a growth factor important in the final differentiation of B-cells into plasma cells and in the pathogenesis of multiple myeloma. To determine whether the receptor for IL-6 may exist as a soluble molecule, RNA was analysed from the transformed B-cell lines U266, CESS and Daudi, from bone marrow from two myeloma patients, and from normal leukocytes. Using polymerase chain reaction, oligonucleotide primers which flank the transmembrane domain were selected to generate a 339 bp fragment. All samples produced equivalent amounts of the expected 339 bp fragment plus a smaller 245 bp fragment except Daudi which exhibited virtual absence of both. Sequence analysis of the smaller fragments from each of the five samples demonstrated the deletion of the entire transmembrane region from codons 356 (G-TG) to 387 (AG-G). The boundaries of this deletion were identical in all cases. Partial sequence analysis of the ligand-binding domain for U266 demonstrated identical sequences for the membrane-bound and soluble forms of the IL-6 receptor cDNAs. In summary, an mRNA which encodes a soluble form of the IL-6 receptor is expressed in both normal and myeloma cells.

KW - interleukin-6

KW - myeloma

KW - soluble receptor

UR - http://www.scopus.com/inward/record.url?scp=0026828552&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026828552&partnerID=8YFLogxK

U2 - 10.1016/1043-4666(92)90043-Q

DO - 10.1016/1043-4666(92)90043-Q

M3 - Article

VL - 4

SP - 96

EP - 100

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 2

ER -