Isolation of Extracellular Vesicles from Stem Cells

Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal Lee Weintraub, Yao Liang Tang

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalMethods in molecular biology (Clifton, N.J.)
Volume1660
DOIs
StatePublished - Jan 1 2017

Keywords

  • Extracellular vesicles
  • Induced pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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