Isolation of Extracellular Vesicles from Stem Cells

Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma, Yutao Liu, Il-man Kim, Neal Lee Weintraub, Yao Liang Tang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transplanted induced pluripotent stem cells (IPSC ) and embryonic stem cells (ESC) exhibit enhanced survival in ischemic tissues and promote survival of neighboring cells via paracrine effects. Recent studies indicate that stem cells can secrete extracellular vesicles (EV), which can shuttle noncoding RNA between cells and facilitate intercellular signaling and communication between donor stem cells and recipient tissues. Direct transplantation of IPSC -derived EV (IPSC -EV) is highly effective at promoting survival and preventing apoptosis of cardiomyocytes in a mouse model of acute myocardial ischemia-reperfusion (MI/R). Here, we describe a feasible protocol to purify EV from cultured IPSC .

Original languageEnglish (US)
Pages (from-to)389-394
Number of pages6
JournalMethods in molecular biology (Clifton, N.J.)
Volume1660
DOIs
StatePublished - Jan 1 2017

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Induced Pluripotent Stem Cells
Stem Cells
Tissue Survival
Untranslated RNA
Myocardial Reperfusion
Embryonic Stem Cells
Cardiac Myocytes
Myocardial Ischemia
Transplantation
Communication
Apoptosis
Extracellular Vesicles

Keywords

  • Extracellular vesicles
  • Induced pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Isolation of Extracellular Vesicles from Stem Cells. / Chen, Zixin; Li, Yongjun; Yu, Hong; Shen, Yan; Ju, Chengwei; Ma, Genshan; Liu, Yutao; Kim, Il-man; Weintraub, Neal Lee; Tang, Yao Liang.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1660, 01.01.2017, p. 389-394.

Research output: Contribution to journalArticle

Chen, Zixin ; Li, Yongjun ; Yu, Hong ; Shen, Yan ; Ju, Chengwei ; Ma, Genshan ; Liu, Yutao ; Kim, Il-man ; Weintraub, Neal Lee ; Tang, Yao Liang. / Isolation of Extracellular Vesicles from Stem Cells. In: Methods in molecular biology (Clifton, N.J.). 2017 ; Vol. 1660. pp. 389-394.
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