Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection

Vita Lan-Ing Goei, Satish Parimoo, Angela Capossela, Thomas W. Chu, Jeffrey R. Gruen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

It has previously been shown that cDNA hybridization selection can identify and recover novel genes from large cloned genomic DNA such as cosmids or YACs. In an effort to identify candidate genes for hemochromatosis, this technique was applied to a 320-kb YAC containing the HLA-A gene. A short fragment cDNA library derived from human duodenum was selected with the YAC DNA. Ten novel gene fragments were isolated, characterized, and localized on the physical map of the YAC.

Original languageEnglish (US)
Pages (from-to)244-251
Number of pages8
JournalAmerican Journal of Human Genetics
Volume54
Issue number2
StatePublished - Feb 1 1994
Externally publishedYes

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Hemochromatosis
Complementary DNA
Genes
Cosmids
HLA-A Antigens
DNA
Gene Library
Duodenum

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection. / Goei, Vita Lan-Ing; Parimoo, Satish; Capossela, Angela; Chu, Thomas W.; Gruen, Jeffrey R.

In: American Journal of Human Genetics, Vol. 54, No. 2, 01.02.1994, p. 244-251.

Research output: Contribution to journalArticle

Goei, Vita Lan-Ing ; Parimoo, Satish ; Capossela, Angela ; Chu, Thomas W. ; Gruen, Jeffrey R. / Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection. In: American Journal of Human Genetics. 1994 ; Vol. 54, No. 2. pp. 244-251.
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