Isolation of Pneumocystis carinii cysts by flow cytometry

Jamie Ann De Stefano, Richard G. Sleight, George F. Babcock, R. Michael Sramkoski, Peter D. Walzer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Pneumocystis carinii cysts were separated as enriched populations from suspensions of lung homogenate obtained from infected rats containing all developmental stages of this organism. Isolation of cyst populations was achieved by incubating filtered lung homogenate with the fluorescent phospholipid analog 1-palmitoyl-2-C6-NBD-phosphatidylcholine. Whereas cysts did not fluorescence as a result of outer-wall restraint of lipid integration, trophozoites and young intermediate stages readily incorporated the fluorescently labeled lipid analog into their outer membrane. The two distinct labeling patterns displayed by cysts and other developmental phases of P. carinii constitute a novel, easy, and reproducible means of isolating cysts from infected lung homogenate by flow cytometry.

Original languageEnglish (US)
Pages (from-to)179-182
Number of pages4
JournalParasitology Research
Volume78
Issue number3
DOIs
StatePublished - Mar 1 1992
Externally publishedYes

Fingerprint

Pneumocystis carinii
flow cytometry
Cysts
Flow Cytometry
lungs
trophozoites
Lung
lipids
phosphatidylcholines
phospholipids
Lipids
Trophozoites
developmental stages
fluorescence
rats
organisms
Population
Phospholipids
Suspensions
Fluorescence

ASJC Scopus subject areas

  • Parasitology

Cite this

De Stefano, J. A., Sleight, R. G., Babcock, G. F., Michael Sramkoski, R., & Walzer, P. D. (1992). Isolation of Pneumocystis carinii cysts by flow cytometry. Parasitology Research, 78(3), 179-182. https://doi.org/10.1007/BF00931724

Isolation of Pneumocystis carinii cysts by flow cytometry. / De Stefano, Jamie Ann; Sleight, Richard G.; Babcock, George F.; Michael Sramkoski, R.; Walzer, Peter D.

In: Parasitology Research, Vol. 78, No. 3, 01.03.1992, p. 179-182.

Research output: Contribution to journalArticle

De Stefano, JA, Sleight, RG, Babcock, GF, Michael Sramkoski, R & Walzer, PD 1992, 'Isolation of Pneumocystis carinii cysts by flow cytometry', Parasitology Research, vol. 78, no. 3, pp. 179-182. https://doi.org/10.1007/BF00931724
De Stefano JA, Sleight RG, Babcock GF, Michael Sramkoski R, Walzer PD. Isolation of Pneumocystis carinii cysts by flow cytometry. Parasitology Research. 1992 Mar 1;78(3):179-182. https://doi.org/10.1007/BF00931724
De Stefano, Jamie Ann ; Sleight, Richard G. ; Babcock, George F. ; Michael Sramkoski, R. ; Walzer, Peter D. / Isolation of Pneumocystis carinii cysts by flow cytometry. In: Parasitology Research. 1992 ; Vol. 78, No. 3. pp. 179-182.
@article{405dc0d1222b43c2a5a44b653cd8fd24,
title = "Isolation of Pneumocystis carinii cysts by flow cytometry",
abstract = "Pneumocystis carinii cysts were separated as enriched populations from suspensions of lung homogenate obtained from infected rats containing all developmental stages of this organism. Isolation of cyst populations was achieved by incubating filtered lung homogenate with the fluorescent phospholipid analog 1-palmitoyl-2-C6-NBD-phosphatidylcholine. Whereas cysts did not fluorescence as a result of outer-wall restraint of lipid integration, trophozoites and young intermediate stages readily incorporated the fluorescently labeled lipid analog into their outer membrane. The two distinct labeling patterns displayed by cysts and other developmental phases of P. carinii constitute a novel, easy, and reproducible means of isolating cysts from infected lung homogenate by flow cytometry.",
author = "{De Stefano}, {Jamie Ann} and Sleight, {Richard G.} and Babcock, {George F.} and {Michael Sramkoski}, R. and Walzer, {Peter D.}",
year = "1992",
month = "3",
day = "1",
doi = "10.1007/BF00931724",
language = "English (US)",
volume = "78",
pages = "179--182",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0932-0113",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Isolation of Pneumocystis carinii cysts by flow cytometry

AU - De Stefano, Jamie Ann

AU - Sleight, Richard G.

AU - Babcock, George F.

AU - Michael Sramkoski, R.

AU - Walzer, Peter D.

PY - 1992/3/1

Y1 - 1992/3/1

N2 - Pneumocystis carinii cysts were separated as enriched populations from suspensions of lung homogenate obtained from infected rats containing all developmental stages of this organism. Isolation of cyst populations was achieved by incubating filtered lung homogenate with the fluorescent phospholipid analog 1-palmitoyl-2-C6-NBD-phosphatidylcholine. Whereas cysts did not fluorescence as a result of outer-wall restraint of lipid integration, trophozoites and young intermediate stages readily incorporated the fluorescently labeled lipid analog into their outer membrane. The two distinct labeling patterns displayed by cysts and other developmental phases of P. carinii constitute a novel, easy, and reproducible means of isolating cysts from infected lung homogenate by flow cytometry.

AB - Pneumocystis carinii cysts were separated as enriched populations from suspensions of lung homogenate obtained from infected rats containing all developmental stages of this organism. Isolation of cyst populations was achieved by incubating filtered lung homogenate with the fluorescent phospholipid analog 1-palmitoyl-2-C6-NBD-phosphatidylcholine. Whereas cysts did not fluorescence as a result of outer-wall restraint of lipid integration, trophozoites and young intermediate stages readily incorporated the fluorescently labeled lipid analog into their outer membrane. The two distinct labeling patterns displayed by cysts and other developmental phases of P. carinii constitute a novel, easy, and reproducible means of isolating cysts from infected lung homogenate by flow cytometry.

UR - http://www.scopus.com/inward/record.url?scp=0026524161&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026524161&partnerID=8YFLogxK

U2 - 10.1007/BF00931724

DO - 10.1007/BF00931724

M3 - Article

VL - 78

SP - 179

EP - 182

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0932-0113

IS - 3

ER -