Isolation of the Nonhistone Proteins of Rat Walker Carcinoma 256. Their Association with Tumor Angiogenesis

Dorothy Tuan Lo, Sandra Smith, Judah Folkman, Ezio Merler

Research output: Contribution to journalArticle

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Abstract

A procedure is described to isolate nonhistone proteins of malignant cell nuclei for the purpose of testing certain of its biologic activities. Walker rat carcinoma 256 cells were disrupted by N2 cavitation, the nuclei isolated by differential centrifugation, the chromatin was purified, and the DNA separated from chromatin protein by chromatography on Bio-Gel A 5m in the presence of 4 M guanidine-HCl. Further separation of histone and nonhistone proteins was achieved with CM-Sephadex C-50, eluting alkylated nonhistones with 0.4 M guanidine-HCl in 0.01 M phosphate buffer and subsequent elution of histones in 2 M guanidine-HCl in 0.1 M acetic acid. Nonhistone and histone proteins were obtained in good yield and satisfactory purity. The histone and nonhistone proteins were characterized by amino acid analysis, macromolecular composition, and gel electrophoresis. A fraction containing tumor nonhistone proteins was mitogenic to endothelial cells, and, when implanted into the rabbit cornea, caused proliferation of vascular endothelium and new blood vessel formation. Tumor angiogenesis factor was thus demonstrated to be associated with a fraction also containing nonhistone proteins of malignant cell nuclei. Histone fractions had no activity in this bioassay.

Original languageEnglish (US)
Pages (from-to)3159-3165
Number of pages7
JournalBiochemistry
Volume12
Issue number17
DOIs
StatePublished - Aug 1 1973

Fingerprint

Walker Carcinoma 256
Rats
Tumors
Histones
Guanidine
Neoplasms
Proteins
Cell Nucleus
Chromatin
Gels
Cells
Centrifugation
Bioassay
Angiogenesis Inducing Agents
Endothelial cells
Vascular Endothelium
Blood vessels
Chromatography
Electrophoresis
Cavitation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Isolation of the Nonhistone Proteins of Rat Walker Carcinoma 256. Their Association with Tumor Angiogenesis. / Tuan Lo, Dorothy; Smith, Sandra; Folkman, Judah; Merler, Ezio.

In: Biochemistry, Vol. 12, No. 17, 01.08.1973, p. 3159-3165.

Research output: Contribution to journalArticle

Tuan Lo, Dorothy ; Smith, Sandra ; Folkman, Judah ; Merler, Ezio. / Isolation of the Nonhistone Proteins of Rat Walker Carcinoma 256. Their Association with Tumor Angiogenesis. In: Biochemistry. 1973 ; Vol. 12, No. 17. pp. 3159-3165.
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