Kinetics of cell adhesion to polymer surfaces

D. R. Absolom, C. Thomson, Lesleyann Hawthorn, W. Zingg, A. W. Neumann

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Results of the kinetics of adhesion of granulocytes as well as fresh and glutaraldehyde‐fixed erythrocytes, suspended in Hanks Balanced Salt Solution (HBSS; pH 7.2, ionic strength of 0.15) to various polymeric substrates are presented. Cell adhesion increases rapidly initially and reaches a plateau value after approximately 30 minutes. There is no evidence for a lag‐time in the onset of cell adhesion, suggesting that electrostatic double‐layer forces are negligible under these experimental conditions. For the experiments in which the cells are suspended in HBSS, which has a surface tension larger than that of the cells, the level of cell adhesion increases with decreasing surface tension of the polymeric substrates. An additional experiment with fresh human granulocytes suspended in HBSS containing 10% dimethyl sulfoxide was also performed. The surface tension of the resulting liquid medium is below that of the cells and the pattern of adhesion is reversed, in agreement with the predictions of a thermodynamic model for cell adhesion. The slightly different behavior of siliconized glass as a substrate is discussed in terms of „screening”.

Original languageEnglish (US)
Pages (from-to)215-229
Number of pages15
JournalJournal of Biomedical Materials Research
Volume22
Issue number3
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Cell adhesion
Cell Adhesion
Polymers
Surface Tension
Surface tension
Kinetics
Substrates
Adhesion
Granulocytes
Dimethyl sulfoxide
Electrostatic force
Dimethyl Sulfoxide
Ionic strength
Screening
Static Electricity
Thermodynamics
Experiments
Osmolar Concentration
Glass
Salts

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Kinetics of cell adhesion to polymer surfaces. / Absolom, D. R.; Thomson, C.; Hawthorn, Lesleyann; Zingg, W.; Neumann, A. W.

In: Journal of Biomedical Materials Research, Vol. 22, No. 3, 01.01.1988, p. 215-229.

Research output: Contribution to journalArticle

Absolom, D. R. ; Thomson, C. ; Hawthorn, Lesleyann ; Zingg, W. ; Neumann, A. W. / Kinetics of cell adhesion to polymer surfaces. In: Journal of Biomedical Materials Research. 1988 ; Vol. 22, No. 3. pp. 215-229.
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