Kinetics of Encephalitozoon spp. infection of human macrophages

Jeffrey L Fischer, Diana Tran, Richard Juneau, Hollie Hale-Donze

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Microsporidia are obligate intracellular, eukaryotic parasites that are known to infect a variety of invertebrate and vertebrate species and have been reported to include a broad range of host specificities for various cell types. Although it is clear that some species of microsporidia have the ability to disseminate, causing multiorgan infections, it is not understood how dissemination occurs. One hypothesis suggests that mononuclear phagocytes engulf the pathogen and migrate to various organs while the parasite persists and proliferates. This implies that microsporidia have developed methods by which to escape intracellular degradation and can, instead, use the host as a source of nourishment and a vehicle for dissemination. In our study, we investigated the infection kinetics of 2 Encephalitozoon spp. known to cause disseminated disease in humans. Using fluorescence and scanning electron microscopy, it was determined that spore adherence to the host was rapid (3-6 hr), as was the uptake and organization of internal parasitophorous vacuoles (24 hr). Furthermore, replication was shown to occur within macrophages at 72 hr, as measured by the bromodeoxyuridine proliferation assay, and the production of mature spores occurred in host cells at 120 hr. Parasitic replication could be reduced by pretreatment of macrophages with interferon-gamma and bacterial lipopolysaccharide.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalJournal of Parasitology
Volume94
Issue number1
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Fingerprint

Encephalitozoon
Microsporidia
spore
parasite
macrophages
Macrophages
Spores
kinetics
host specificity
Parasites
spores
Infection
infection
parasites
vertebrate
fluorescence
pathogen
invertebrate
Host Specificity
phagocytes

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Kinetics of Encephalitozoon spp. infection of human macrophages. / Fischer, Jeffrey L; Tran, Diana; Juneau, Richard; Hale-Donze, Hollie.

In: Journal of Parasitology, Vol. 94, No. 1, 01.02.2008, p. 169-175.

Research output: Contribution to journalArticle

Fischer, Jeffrey L ; Tran, Diana ; Juneau, Richard ; Hale-Donze, Hollie. / Kinetics of Encephalitozoon spp. infection of human macrophages. In: Journal of Parasitology. 2008 ; Vol. 94, No. 1. pp. 169-175.
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