Abstract
Microsporidia are obligate intracellular, eukaryotic parasites that are known to infect a variety of invertebrate and vertebrate species and have been reported to include a broad range of host specificities for various cell types. Although it is clear that some species of microsporidia have the ability to disseminate, causing multiorgan infections, it is not understood how dissemination occurs. One hypothesis suggests that mononuclear phagocytes engulf the pathogen and migrate to various organs while the parasite persists and proliferates. This implies that microsporidia have developed methods by which to escape intracellular degradation and can, instead, use the host as a source of nourishment and a vehicle for dissemination. In our study, we investigated the infection kinetics of 2 Encephalitozoon spp. known to cause disseminated disease in humans. Using fluorescence and scanning electron microscopy, it was determined that spore adherence to the host was rapid (3-6 hr), as was the uptake and organization of internal parasitophorous vacuoles (24 hr). Furthermore, replication was shown to occur within macrophages at 72 hr, as measured by the bromodeoxyuridine proliferation assay, and the production of mature spores occurred in host cells at 120 hr. Parasitic replication could be reduced by pretreatment of macrophages with interferon-gamma and bacterial lipopolysaccharide.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 169-175 |
| Number of pages | 7 |
| Journal | Journal of Parasitology |
| Volume | 94 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Parasitology
- Ecology, Evolution, Behavior and Systematics
Cite this
Kinetics of Encephalitozoon spp. infection of human macrophages. / Fischer, Jeffrey L; Tran, Diana; Juneau, Richard; Hale-Donze, Hollie.
In: Journal of Parasitology, Vol. 94, No. 1, 01.02.2008, p. 169-175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinetics of Encephalitozoon spp. infection of human macrophages
AU - Fischer, Jeffrey L
AU - Tran, Diana
AU - Juneau, Richard
AU - Hale-Donze, Hollie
PY - 2008/2/1
Y1 - 2008/2/1
N2 - Microsporidia are obligate intracellular, eukaryotic parasites that are known to infect a variety of invertebrate and vertebrate species and have been reported to include a broad range of host specificities for various cell types. Although it is clear that some species of microsporidia have the ability to disseminate, causing multiorgan infections, it is not understood how dissemination occurs. One hypothesis suggests that mononuclear phagocytes engulf the pathogen and migrate to various organs while the parasite persists and proliferates. This implies that microsporidia have developed methods by which to escape intracellular degradation and can, instead, use the host as a source of nourishment and a vehicle for dissemination. In our study, we investigated the infection kinetics of 2 Encephalitozoon spp. known to cause disseminated disease in humans. Using fluorescence and scanning electron microscopy, it was determined that spore adherence to the host was rapid (3-6 hr), as was the uptake and organization of internal parasitophorous vacuoles (24 hr). Furthermore, replication was shown to occur within macrophages at 72 hr, as measured by the bromodeoxyuridine proliferation assay, and the production of mature spores occurred in host cells at 120 hr. Parasitic replication could be reduced by pretreatment of macrophages with interferon-gamma and bacterial lipopolysaccharide.
AB - Microsporidia are obligate intracellular, eukaryotic parasites that are known to infect a variety of invertebrate and vertebrate species and have been reported to include a broad range of host specificities for various cell types. Although it is clear that some species of microsporidia have the ability to disseminate, causing multiorgan infections, it is not understood how dissemination occurs. One hypothesis suggests that mononuclear phagocytes engulf the pathogen and migrate to various organs while the parasite persists and proliferates. This implies that microsporidia have developed methods by which to escape intracellular degradation and can, instead, use the host as a source of nourishment and a vehicle for dissemination. In our study, we investigated the infection kinetics of 2 Encephalitozoon spp. known to cause disseminated disease in humans. Using fluorescence and scanning electron microscopy, it was determined that spore adherence to the host was rapid (3-6 hr), as was the uptake and organization of internal parasitophorous vacuoles (24 hr). Furthermore, replication was shown to occur within macrophages at 72 hr, as measured by the bromodeoxyuridine proliferation assay, and the production of mature spores occurred in host cells at 120 hr. Parasitic replication could be reduced by pretreatment of macrophages with interferon-gamma and bacterial lipopolysaccharide.
UR - http://www.scopus.com/inward/record.url?scp=40949163497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40949163497&partnerID=8YFLogxK
U2 - 10.1645/GE-1303.1
DO - 10.1645/GE-1303.1
M3 - Article
C2 - 18372637
AN - SCOPUS:40949163497
VL - 94
SP - 169
EP - 175
JO - Journal of Parasitology
JF - Journal of Parasitology
SN - 0022-3395
IS - 1
ER -