Kinetics of iron oxidation upon polyphenol binding

Nathan R. Perron, Hsiao C. Wang, Sean N. Deguire, Michael Jenkins, Mereze Lawson, Julia L. Brumaghim

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Polyphenol prevention of iron-mediated DNA damage occurs primarily through iron binding. Once bound, iron in the Fe2+-polyphenol complex autooxidizes to Fe3+ in the presence of O2. To determine the correlation between the rate of Fe2+-polyphenol autooxidation and polyphenol antioxidant ability, kinetic studies at pH = 6.0 in the presence of oxygen were performed using UV-vis spectrophotometry. Initial rates of iron-polyphenol complex oxidation for epigallocatechin gallate (EGCG), methyl-3,4,5-trihydroxybenzoate (MEGA), gallic acid (GA), epicatechin (EC), and methyl-3,4-dihydroxybenzoate (MEPCA) were in the range of 0.14-6.7 min -1. Polyphenols with gallol groups have faster rates of iron oxidation than their catechol analogs, suggesting that stronger iron binding results in faster iron oxidation. Concentrations of polyphenol, Fe2+, and O2 were varied to investigate the dependence of the Fe 2+-polyphenol autooxidation on these reactants for MEGA and MEPCA. For these analogous gallate and catecholate complexes of Fe2+, iron oxidation reactions were first order in Fe2+, polyphenol, and O 2, but gallate complexes show saturation behavior at much lower Fe2+ concentrations. Thus, gallol-containing polyphenols promote iron oxidation at a significantly faster rate than analogous catechol-containing compounds, and iron oxidation rate also correlates strongly with polyphenol inhibition of DNA damage for polyphenol compounds with a single iron-binding moiety.

Original languageEnglish (US)
Pages (from-to)9982-9987
Number of pages6
JournalDalton Transactions
Volume39
Issue number41
DOIs
StatePublished - Nov 7 2010

ASJC Scopus subject areas

  • Inorganic Chemistry

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    Perron, N. R., Wang, H. C., Deguire, S. N., Jenkins, M., Lawson, M., & Brumaghim, J. L. (2010). Kinetics of iron oxidation upon polyphenol binding. Dalton Transactions, 39(41), 9982-9987. https://doi.org/10.1039/c0dt00752h