Kinetics of iron oxidation upon polyphenol binding

Nathan R. Perron, Hsiao C. Wang, Sean N. Deguire, Michael Jenkins, Mereze Lawson, Julia L. Brumaghim

    Research output: Contribution to journalArticlepeer-review

    110 Scopus citations

    Abstract

    Polyphenol prevention of iron-mediated DNA damage occurs primarily through iron binding. Once bound, iron in the Fe2+-polyphenol complex autooxidizes to Fe3+ in the presence of O2. To determine the correlation between the rate of Fe2+-polyphenol autooxidation and polyphenol antioxidant ability, kinetic studies at pH = 6.0 in the presence of oxygen were performed using UV-vis spectrophotometry. Initial rates of iron-polyphenol complex oxidation for epigallocatechin gallate (EGCG), methyl-3,4,5-trihydroxybenzoate (MEGA), gallic acid (GA), epicatechin (EC), and methyl-3,4-dihydroxybenzoate (MEPCA) were in the range of 0.14-6.7 min -1. Polyphenols with gallol groups have faster rates of iron oxidation than their catechol analogs, suggesting that stronger iron binding results in faster iron oxidation. Concentrations of polyphenol, Fe2+, and O2 were varied to investigate the dependence of the Fe 2+-polyphenol autooxidation on these reactants for MEGA and MEPCA. For these analogous gallate and catecholate complexes of Fe2+, iron oxidation reactions were first order in Fe2+, polyphenol, and O 2, but gallate complexes show saturation behavior at much lower Fe2+ concentrations. Thus, gallol-containing polyphenols promote iron oxidation at a significantly faster rate than analogous catechol-containing compounds, and iron oxidation rate also correlates strongly with polyphenol inhibition of DNA damage for polyphenol compounds with a single iron-binding moiety.

    Original languageEnglish (US)
    Pages (from-to)9982-9987
    Number of pages6
    JournalDalton Transactions
    Volume39
    Issue number41
    DOIs
    StatePublished - Nov 7 2010

    ASJC Scopus subject areas

    • Inorganic Chemistry

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