@article{bc55dbd02bc4429ea6fe00add43a2e69,
title = "Kv1.3 Channel Gene-Targeted Deletion Produces {"}Super-Smeller Mice{"} with Altered Glomeruli, Interacting Scaffolding Proteins, and Biophysics",
abstract = "Mice with gene-targeted deletion of the Kv1.3 channel were generated to study its role in olfactory function. Potassium currents in olfactory bulb mitral cells from Kv1.3 null mice have slow inactivation kinetics, a modified voltage dependence, and a dampened C-type inactivation and fail to be modulated by activators of receptor tyrosine signaling cascades. Kv1.3 deletion increases expression of scaffolding proteins that normally regulate the channel through protein-protein interactions. Kv1.3-/- mice have a 1,000- to 10,000-fold lower threshold for detection of odors and an increased ability to discriminate between odorants. In accordance with this heightened sense of smell, Kv1.3-/- mice have glomeruli or olfactory coding units that are smaller and more numerous than those of wild-type mice. These data suggest that Kv1.3 plays a far more reaching role in signal transduction, development, and olfactory coding than that of the classically defined role of a potassium channel - to shape excitability by influencing membrane potential.",
author = "Fadool, {D. A.} and K. Tucker and R. Perkins and G. Fasciani and Thompson, {R. N.} and Parsons, {A. D.} and Overton, {J. M.} and Koni, {P. A.} and Flavell, {R. A.} and Kaczmarek, {L. K.}",
note = "Funding Information: We would like to thank the following undergraduate volunteers for assistance with behavioral experiments, mouse colony maintenance, and routine genotyping: Mr. Matt Edsall, Ms. Dietra Haynes, Mr. Dan Otten, Mr. Grant Richards, and Mr. Chad Thorson. We would like to thank Mr. David Watson and XiXi Jia for technical assistance with cryosectioning; Dr. Michael Meredith and Ms. Jessica Brann for histological assistance; Ms. Rani Dhanarajan for assistance with RNA handling; Ms. Jill Scarbourough for technical assistance; and Drs. Marc Freeman and James Fadool for access to image analysis and photomicroscopy equipment, respectively. We are grateful to Drs. Ben Strowbridge, James Fadool, Laura Blakemore, and Paul Trombley for very helpful discussions; and Drs. Hanno Wurbel and Lynn Hyde for valuable insight into the design of our mouse behavioral assays. This work was supported by NIH grants DC03387 (NIDCD, D.A.F.) and DC01919 (NIDCD, L.K.K). R.A.F. is an investigator of the Howard Hughes Medical Institute. ",
year = "2004",
month = feb,
day = "5",
doi = "10.1016/S0896-6273(03)00844-4",
language = "English (US)",
volume = "41",
pages = "389--404",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",
}