L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury

Terrence M. Donohue, Tiana Vitula Curry-McCoy, Sandra L. Todero, Ronda L. White, Kusum K. Kharbanda, Amin A. Nanji, Natalia A. Osna

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: L-Buthionine (S,R) sulfoximine (BSO) is an inhibitor of glutathione biosynthesis and has been used as an effective means of depleting glutathione from cells and tissues. Here we investigated whether treatment with BSO enhanced ethanol-induced liver injury in mice. Methods: Female C57Bl/6 mice were pair fed with control and ethanol-containing liquid diets in which ethanol was 29.2% of total calories. During the final 7 days of pair feeding, groups of control-fed and ethanol-fed mice were given 0, 5 or 7.6 mM BSO in the liquid diets. Results: Compared with controls, ethanol given alone decreased total liver glutathione. This effect was exacerbated in mice given ethanol with 7.6 mM BSO, causing a 72% decline in hepatic glutathione. While ethanol alone caused no decrease in mitochondrial glutathione, inclusion of 7.6 mM BSO caused a 2-fold decline compared with untreated controls. l-Buthionine (S,R) sulfoximine did not affect ethanol consumption, but serum ethanol levels in BSO-treated mice were nearly 6-fold lower than in mice given ethanol alone. The latter decline in serum ethanol was associated with a significant elevation in the specific activities of cytochrome P450 2E1 and alcohol dehydrogenase in livers of BSO-treated animals. Ethanol consumption caused a 3.5-fold elevation in serum alanine aminotransferase levels but the enzyme fell to control levels when BSO was included in the diet. l-Buthionine (S,R) sulfoximine administration also attenuated ethanol-induced steatosis, prevented the leakage of lysosomal cathepsins into the cytosol, and prevented the ethanol-elicited decline in proteasome activity. Conclusions: l-Buthionine (S,R) sulfoximine, administered with ethanol, significantly depleted hepatic glutathione, compared with controls. However, despite the decrease in hepatic antioxidant levels, liver injury by ethanol was alleviated, due, in part, to a BSO-elicited acceleration of ethanol metabolism.

Original languageEnglish (US)
Pages (from-to)1053-1060
Number of pages8
JournalAlcoholism: Clinical and Experimental Research
Volume31
Issue number6
DOIs
StatePublished - Jan 1 2007

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Liver
Glutathione
Ethanol
Wounds and Injuries
Nutrition
buthionine
Diet
Serum
Cathepsins
Cytochrome P-450 CYP2E1
Alcohol Dehydrogenase
Biosynthesis
Level control
Liquids
Proteasome Endopeptidase Complex
Alanine Transaminase
Metabolism
Cytosol
Animals

Keywords

  • Alcohol Dehydrogenase
  • Cytochrome P450 2E1
  • Ethanol
  • Glutathione
  • Oxidative Stress

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury. / Donohue, Terrence M.; Curry-McCoy, Tiana Vitula; Todero, Sandra L.; White, Ronda L.; Kharbanda, Kusum K.; Nanji, Amin A.; Osna, Natalia A.

In: Alcoholism: Clinical and Experimental Research, Vol. 31, No. 6, 01.01.2007, p. 1053-1060.

Research output: Contribution to journalArticle

Donohue, Terrence M. ; Curry-McCoy, Tiana Vitula ; Todero, Sandra L. ; White, Ronda L. ; Kharbanda, Kusum K. ; Nanji, Amin A. ; Osna, Natalia A. / L-Buthionine (S,R) sulfoximine depletes hepatic glutathione but protects against ethanol-induced liver injury. In: Alcoholism: Clinical and Experimental Research. 2007 ; Vol. 31, No. 6. pp. 1053-1060.
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abstract = "Background: L-Buthionine (S,R) sulfoximine (BSO) is an inhibitor of glutathione biosynthesis and has been used as an effective means of depleting glutathione from cells and tissues. Here we investigated whether treatment with BSO enhanced ethanol-induced liver injury in mice. Methods: Female C57Bl/6 mice were pair fed with control and ethanol-containing liquid diets in which ethanol was 29.2{\%} of total calories. During the final 7 days of pair feeding, groups of control-fed and ethanol-fed mice were given 0, 5 or 7.6 mM BSO in the liquid diets. Results: Compared with controls, ethanol given alone decreased total liver glutathione. This effect was exacerbated in mice given ethanol with 7.6 mM BSO, causing a 72{\%} decline in hepatic glutathione. While ethanol alone caused no decrease in mitochondrial glutathione, inclusion of 7.6 mM BSO caused a 2-fold decline compared with untreated controls. l-Buthionine (S,R) sulfoximine did not affect ethanol consumption, but serum ethanol levels in BSO-treated mice were nearly 6-fold lower than in mice given ethanol alone. The latter decline in serum ethanol was associated with a significant elevation in the specific activities of cytochrome P450 2E1 and alcohol dehydrogenase in livers of BSO-treated animals. Ethanol consumption caused a 3.5-fold elevation in serum alanine aminotransferase levels but the enzyme fell to control levels when BSO was included in the diet. l-Buthionine (S,R) sulfoximine administration also attenuated ethanol-induced steatosis, prevented the leakage of lysosomal cathepsins into the cytosol, and prevented the ethanol-elicited decline in proteasome activity. Conclusions: l-Buthionine (S,R) sulfoximine, administered with ethanol, significantly depleted hepatic glutathione, compared with controls. However, despite the decrease in hepatic antioxidant levels, liver injury by ethanol was alleviated, due, in part, to a BSO-elicited acceleration of ethanol metabolism.",
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AU - Todero, Sandra L.

AU - White, Ronda L.

AU - Kharbanda, Kusum K.

AU - Nanji, Amin A.

AU - Osna, Natalia A.

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