Label-free detection of proteins from self-assembled protein-silver nanoparticle structures using surface-enhanced raman scattering

Mehmet Kahraman, Ilknur Sur, Mustafa Çulha

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Proteins are one of the most versatile groups of molecules with vital functional roles in living systems. Their enormous diversity and structural flexibility make the detection of these molecules a challenging task. A simple and sensitive label-free protein detection method based on assembly of proteins and colloidal silver nanoparticles (AgNPs) on surfaces and surface-enhanced Raman scattering (SERS) is reported. The SERS spectra from the assembled AgNP/protein films show excellent reproducibility and high quality regardless of the proteins' charge status and size. A detection limit down to 0.5 μg/mL for three acidic proteins; BSA, catalase and pepsin, and three basic proteins; cytochrome c, avidin and lysozyme, is easily achieved. The minimum improvement in detection limit is more than 1 order of magnitude compared to the previously reported detection limits using the technique and the approach has the potential for label-free protein detection and identification.

Original languageEnglish (US)
Pages (from-to)7596-7602
Number of pages7
JournalAnalytical Chemistry
Volume82
Issue number18
DOIs
StatePublished - Sep 15 2010
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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