Label-free DNA methylation analysis using the optofluidic ring resonator sensor

Jonathan D. Suter, Daniel J. Howard, Charles W. Caldwell, Huidong Shi, Xudong Fan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for the purpose of analyzing the degree of methylation in sample oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, is prone to abnormal behavior that may lead to uncontrolled suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool to explore and monitor the degree of methylation in DNA. Two different approaches are explored, using either bisulfite modification or immunoprecipitation. The methods are compared and the signal response for both methods is characterized.

Original languageEnglish (US)
Title of host publicationPhotonic Microdevices/Microstructures for Sensing
DOIs
StatePublished - Sep 14 2009
EventPhotonic Microdevices/Microstructures for Sensing - Orlando, FL, United States
Duration: Apr 16 2009Apr 17 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7322
ISSN (Print)0277-786X

Other

OtherPhotonic Microdevices/Microstructures for Sensing
CountryUnited States
CityOrlando, FL
Period4/16/094/17/09

Keywords

  • Biophotonics
  • Biosensors
  • DNA methylation
  • Optical ring resonators
  • Whispering gallery modes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Label-free DNA methylation analysis using the optofluidic ring resonator sensor'. Together they form a unique fingerprint.

  • Cite this

    Suter, J. D., Howard, D. J., Caldwell, C. W., Shi, H., & Fan, X. (2009). Label-free DNA methylation analysis using the optofluidic ring resonator sensor. In Photonic Microdevices/Microstructures for Sensing [732208] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7322). https://doi.org/10.1117/12.818562