Label-free DNA methylation analysis using the optofluidic ring resonator sensor

Jonathan D. Suter, Daniel J. Howard, Charles W. Caldwell, Huidong Shi, Xudong Fan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for the purpose of analyzing the degree of methylation in sample oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, is prone to abnormal behavior that may lead to uncontrolled suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool to explore and monitor the degree of methylation in DNA. Two different approaches are explored, using either bisulfite modification or immunoprecipitation. The methods are compared and the signal response for both methods is characterized.

Original languageEnglish (US)
Title of host publicationPhotonic Microdevices/Microstructures for Sensing
DOIs
StatePublished - Sep 14 2009
EventPhotonic Microdevices/Microstructures for Sensing - Orlando, FL, United States
Duration: Apr 16 2009Apr 17 2009

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7322
ISSN (Print)0277-786X

Other

OtherPhotonic Microdevices/Microstructures for Sensing
CountryUnited States
CityOrlando, FL
Period4/16/094/17/09

Fingerprint

Optofluidics
Ring Resonator
methylation
Methylation
Labels
Resonators
deoxyribonucleic acid
resonators
fluidics
Fluidics
Sensor
rings
sensors
Sensors
Proliferation
Metabolism
Cancer
Monitor
oligonucleotides
Oligonucleotides

Keywords

  • Biophotonics
  • Biosensors
  • DNA methylation
  • Optical ring resonators
  • Whispering gallery modes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Suter, J. D., Howard, D. J., Caldwell, C. W., Shi, H., & Fan, X. (2009). Label-free DNA methylation analysis using the optofluidic ring resonator sensor. In Photonic Microdevices/Microstructures for Sensing [732208] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7322). https://doi.org/10.1117/12.818562

Label-free DNA methylation analysis using the optofluidic ring resonator sensor. / Suter, Jonathan D.; Howard, Daniel J.; Caldwell, Charles W.; Shi, Huidong; Fan, Xudong.

Photonic Microdevices/Microstructures for Sensing. 2009. 732208 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7322).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Suter, JD, Howard, DJ, Caldwell, CW, Shi, H & Fan, X 2009, Label-free DNA methylation analysis using the optofluidic ring resonator sensor. in Photonic Microdevices/Microstructures for Sensing., 732208, Proceedings of SPIE - The International Society for Optical Engineering, vol. 7322, Photonic Microdevices/Microstructures for Sensing, Orlando, FL, United States, 4/16/09. https://doi.org/10.1117/12.818562
Suter JD, Howard DJ, Caldwell CW, Shi H, Fan X. Label-free DNA methylation analysis using the optofluidic ring resonator sensor. In Photonic Microdevices/Microstructures for Sensing. 2009. 732208. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.818562
Suter, Jonathan D. ; Howard, Daniel J. ; Caldwell, Charles W. ; Shi, Huidong ; Fan, Xudong. / Label-free DNA methylation analysis using the optofluidic ring resonator sensor. Photonic Microdevices/Microstructures for Sensing. 2009. (Proceedings of SPIE - The International Society for Optical Engineering).
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