TY - GEN
T1 - Label-free ferrohydrodynamic cell separation of circulating tumor cells
AU - Zhao, Wujun
AU - Cheng, Rui
AU - Jenkins, Brittany D.
AU - Davis, Melissa B.
AU - Kavuri, Sravan K.
AU - Hao, Zhonglin
AU - Schroeder, Carsten
AU - Mao, Leidong
PY - 2020/1/1
Y1 - 2020/1/1
N2 - We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.
AB - We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.
KW - Circulating tumor cell
KW - Ferrofluid
KW - Ferrohydrodynamic cell separation
UR - http://www.scopus.com/inward/record.url?scp=85079613020&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079613020&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 868
EP - 869
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -