Label-free ferrohydrodynamic cell separation of circulating tumor cells

Wujun Zhao; Rui Cheng; Brittany D. Jenkins; Melissa B. Davis; Sravan K. Kavuri; Zhonglin Hao; Carsten Schroeder; Leidong Mao

Label-free ferrohydrodynamic cell separation of circulating tumor cells

Wujun Zhao, Rui Cheng, Brittany D. Jenkins, Melissa B. Davis, Sravan K. Kavuri, Zhonglin Hao, Carsten Schroeder, Leidong Mao

Pathology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.

Original language	English (US)
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Publisher	Chemical and Biological Microsystems Society
Pages	868-869
Number of pages	2
ISBN (Electronic)	9780692941836
State	Published - Jan 1 2020
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: Oct 22 2017 → Oct 26 2017

Publication series

Name	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/Territory	United States
City	Savannah
Period	10/22/17 → 10/26/17

Keywords

Circulating tumor cell
Ferrofluid
Ferrohydrodynamic cell separation

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Zhao, W., Cheng, R., Jenkins, B. D., Davis, M. B., Kavuri, S. K., Hao, Z., Schroeder, C., & Mao, L. (2020). Label-free ferrohydrodynamic cell separation of circulating tumor cells. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 868-869). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.

Label-free ferrohydrodynamic cell separation of circulating tumor cells. / Zhao, Wujun; Cheng, Rui; Jenkins, Brittany D. et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. p. 868-869 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Zhao, W, Cheng, R, Jenkins, BD, Davis, MB, Kavuri, SK, Hao, Z, Schroeder, C & Mao, L 2020, Label-free ferrohydrodynamic cell separation of circulating tumor cells. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Chemical and Biological Microsystems Society, pp. 868-869, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, United States, 10/22/17.

Zhao W, Cheng R, Jenkins BD, Davis MB, Kavuri SK, Hao Z et al. Label-free ferrohydrodynamic cell separation of circulating tumor cells. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society. 2020. p. 868-869. (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

Zhao, Wujun ; Cheng, Rui ; Jenkins, Brittany D. et al. / Label-free ferrohydrodynamic cell separation of circulating tumor cells. 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2020. pp. 868-869 (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017).

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title = "Label-free ferrohydrodynamic cell separation of circulating tumor cells",

abstract = "We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.",

keywords = "Circulating tumor cell, Ferrofluid, Ferrohydrodynamic cell separation",

author = "Wujun Zhao and Rui Cheng and Jenkins, {Brittany D.} and Davis, {Melissa B.} and Kavuri, {Sravan K.} and Zhonglin Hao and Carsten Schroeder and Leidong Mao",

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T1 - Label-free ferrohydrodynamic cell separation of circulating tumor cells

AU - Zhao, Wujun

AU - Cheng, Rui

AU - Jenkins, Brittany D.

AU - Davis, Melissa B.

AU - Kavuri, Sravan K.

AU - Hao, Zhonglin

AU - Schroeder, Carsten

AU - Mao, Leidong

PY - 2020/1/1

Y1 - 2020/1/1

N2 - We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.

AB - We report a label-free ferrohydrodynamic cell separation (FCS) technology that is capable of high-throughput (6 mL/h), high recovery rate (92.9%), and biocompatible enrichment of circulating tumor cells (CTCs) from cancer patient blood, by systematically investigating the device operating parameters. We show cancer cells have excellent short-term viability, unaffected biomarker expressions, and ability to proliferate to confluence after FCS process. We apply FCS to successfully enrich CTCs from blood of non-small cell lung cancer (NSCLC) patients.

KW - Circulating tumor cell

KW - Ferrofluid

KW - Ferrohydrodynamic cell separation

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M3 - Conference contribution

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SP - 868

EP - 869

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

PB - Chemical and Biological Microsystems Society

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Y2 - 22 October 2017 through 26 October 2017

ER -

Label-free ferrohydrodynamic cell separation of circulating tumor cells

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this