TY - JOUR
T1 - Lack of apparent neurological abnormalities in rabbits sensitized by gangliosides
AU - Dasgupta, Somsankar
AU - Li, Donna
AU - Yu, Robert K
N1 - Funding Information:
This work was supported by a USPHS Grant NS26994. We thank Dr. Alex Chiu for assistance in cardiac puncture, and Dr. Nancy Rodriguez and Dr. Pauline M. Rakich for their help in the clinical and histopathological examinations of the experimental animals.
PY - 2004/11
Y1 - 2004/11
N2 - Two very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund's adjuvant. The antisera were prepared over a period of 6 months with repeated injections of the ganglioside suspension, followed by an intravenous injection of the purified ganglioside solution, and collecting the serum (approximately 50 ml) at defined time intervals. The GM1-antibody, thus prepared, showed a cross reactivity toward GD1b and asialo-GM1 (GA1), while the GD1a-antibody reacted with GD1a, GM1 and GA1 and GD1b as determined by immuno-overlay and ELISA methods. The titer for GM1 antiserum, determined by ELISA, was greater than 1/10,000 dilution while the titer for GD1a antibody was greater than 1/5,000 dilution. No neurological or behavioral abnormality was observed during the period of antiserum production. To evaluate any likely pathological damage caused by such a high titer ganglioside-antibody, autopsy of CNS as well PNS tissues from the rabbits were carried out after the final bleeding. No obvious pathological changes, including demyelination, were noted in any of the four rabbits. These observations cast doubt as to the direct effect of anti-ganglioside antibody induced neurological and pathological disorders.
AB - Two very high titer polyclonal antibodies against two ganglioside antigens, GM1 and GD1a, have been raised in New Zealand white rabbits using a homogeneous suspension of the highly purified antigens in Keyhole Limpet Hemocyanin and Freund's adjuvant. The antisera were prepared over a period of 6 months with repeated injections of the ganglioside suspension, followed by an intravenous injection of the purified ganglioside solution, and collecting the serum (approximately 50 ml) at defined time intervals. The GM1-antibody, thus prepared, showed a cross reactivity toward GD1b and asialo-GM1 (GA1), while the GD1a-antibody reacted with GD1a, GM1 and GA1 and GD1b as determined by immuno-overlay and ELISA methods. The titer for GM1 antiserum, determined by ELISA, was greater than 1/10,000 dilution while the titer for GD1a antibody was greater than 1/5,000 dilution. No neurological or behavioral abnormality was observed during the period of antiserum production. To evaluate any likely pathological damage caused by such a high titer ganglioside-antibody, autopsy of CNS as well PNS tissues from the rabbits were carried out after the final bleeding. No obvious pathological changes, including demyelination, were noted in any of the four rabbits. These observations cast doubt as to the direct effect of anti-ganglioside antibody induced neurological and pathological disorders.
KW - Antibody
KW - Glycosphingolipid
KW - autoimmunity
KW - neurological disorders
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U2 - 10.1007/s11064-004-6888-7
DO - 10.1007/s11064-004-6888-7
M3 - Article
C2 - 15662849
AN - SCOPUS:10844221533
SN - 0364-3190
VL - 29
SP - 2147
EP - 2152
JO - Neurochemical Research
JF - Neurochemical Research
IS - 11 SPEC. ISS.
ER -