Lack of Change in MnSOD During Ischemia/Reperfusion of Isolated Rat Heart

Ramiah Subramanian, Anton Volovsek, Ye Shih Ho

Research output: Contribution to journalArticle

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Abstract

Decreased endogenous superoxide dismutase (SOD) activity has been implicated in free radical-mediated reperfusion injury of the ischemic myocardium. Antioxidant enzymes have been added to the modalities of reperfusion therapy of acute myocardial infarction based on this observation. We measured the content of MnSOD specific protein, activity of Mn and Cu, ZnSODs, and MnSOD mRNA in the working isolated rat heart subjected to various durations of ischemia and reperfusion. Recovery of mechanical function was monitored and lactate and lactic dehydrogenase released in the coronary effluent before and after ischemia were measured. In this model with reversible or irreversible myocardial injury, we noted no change in the myocardial MnSOD specific protein content and, contrary to some previous observations, no change in the activity levels of Mn or Cu, ZnSODs. Our results suggest that free radical-mediated damage in the heart during ischemia and reperfusion is probably not due to impaired activity or degradation of native SODs.

Original languageEnglish (US)
Article number71131
Pages (from-to)1179-1186
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume25
Issue number10
DOIs
StatePublished - Jan 1 1993

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Keywords

  • Cardiac output
  • Ischemia
  • Lactate
  • Lactic dehydrogenase
  • Myocardium
  • Rat
  • Reperfusion
  • Superoxide dismutase

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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