Lactate and the effects of exercise on testosterone secretion: Evidence for the involvement of a cAMP-mediated mechanism

L. U. Shin-Shan, Chin Pang Lau, Yuh Fan Tung, Seng Wong Huang, Yen-Hao Chen, Hsi Chang Shih, Shiow Chwen Tsai, L. U. Chien-Chen, Shyi W.U. Wang, Jing Jong Chen, Eillen Jea Chien, Chau Heng Chien, Paulus S. Wang

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Abstract

The effects of swimming and lactate on the release of testosterone were examined in male rats. During in vivo experiments, male rats were catheterized via the right jugular vein and blood was collected at 0, 10, 15, 30, and 60 min following the exercise, or they were catheterized via the right jugular vein and the left femoral vein and blood was collected at 0, 2, 5, 10, 15, 30, 60, and 120 min after a 10-min infusion of lactate (13 mg · kg-1 · min-1). Trunk blood and blood from the testicular vein were also collected after 10 min of swimming or water immersion. In an in vitro experiment, testicular fragments were challenged with lactate (0.01-10 mM) and/or human chorionic gonadotropin (hCG; 0.5 IU · mL-1), and the mediobasal hypothalamus (MBH) was challenged with lactate (8 mM). The post- exercise levels of plasma lactate and testosterone at 10, 15, and 30 min were higher than resting levels. Plasma luteinizing hormone (LH) was increased following 30 min of swimming. Administration of lactate or hCG increased in a dose dependent manner testicular cyclic adenosine 3':5' monophosphate (cAMP) and testosterone release. Plasma testosterone increased after swimming and lactate infusion. Incubation of MBH with lecture increased the gonadotropin- releasing hormone (GnRH) level in the medium. These results suggest that the increased plasma testosterone levels in male rats during exercise is at least partially a result of a direct and LH-independent stimulatory effect of lactate on the secretion of testosterone by increasing testicular cAMP production. Swim-elevated plasma LH may be a result of a rise of GnRH caused by lactate.

Original languageEnglish (US)
Pages (from-to)1048-1054
Number of pages7
JournalMedicine and Science in Sports and Exercise
Volume29
Issue number8
DOIs
StatePublished - Sep 1 1997

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Keywords

  • GnRH
  • LH
  • Lactate
  • Swim

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Shin-Shan, L. U., Lau, C. P., Tung, Y. F., Huang, S. W., Chen, Y-H., Shih, H. C., Tsai, S. C., Chien-Chen, L. U., Wang, S. W. U., Chen, J. J., Chien, E. J., Chien, C. H., & Wang, P. S. (1997). Lactate and the effects of exercise on testosterone secretion: Evidence for the involvement of a cAMP-mediated mechanism. Medicine and Science in Sports and Exercise, 29(8), 1048-1054. https://doi.org/10.1097/00005768-199708000-00010