Lactate stimulates progesterone secretion via an increase in cAMP production in exercised female rats

Shin Shan Lu, Chin Pang Lau, Yuh Fan Tung, Seng Wong Huang, Yen Hao Chen, Hsi Chang Shih, Shiow Chwen Tsai, Chien Chen Lu, Shyi Wu Wang, Jing Jong Chen, Eillen Jea Chien, Chau Heng Chien, Paulus S. Wang

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Abstract

The effect of exercise on the production of ovarian progesterone was examined in female rats. During in vivo experiments, diestrous rats were catheterized via the right jugular vein (RJV), and blood samples were collected before and after 10, 15, 30, and 60 min of swimming. In addition, blood samples were collected from the RJV before and 2, 5, 10, 15, 30, 60, and 120 min after 10 min of infusion of lactate (13 mg · kg-1 · min-1) through the left femoral vein. To explore if lactate modulates progesterone secretion by acting directly on rat ovary or on anterior pituitary gland (AP), an in vitro experiment that mimicked the in vivo condition was performed. The ovarian tissue was challenged with lactate (0.01-10 mM) or porcine follicle-stimulating hormone (1 μg/ml) and 3-isobutyl-1- methylxanthine (1 mM) for 60 min, and the AP was challenged with lactate ranging from 0.1 to 10 mM or 10 nM gonadotropin-releasing hormone for 30 min. The postexercise levels of plasma glucose, lactate, and progesterone at 10, 15, and 30 min were significantly higher than the corresponding basal levels. Plasma luteinizing hormome (LH) did not change after exercise. An elevation of plasmal lactate and progesterone was found at 15 and 30 min subsequent to 10 min of infusion of lactate. Lactate ranging from 0.01 to 10 mM significantly increased ovarian adenosine 3',5'-cyclic monophosphate (cAMP) and progesterone production in a dose-dependent manner. LH concentration in plasma was not changed subsequent to lactate infusion. LH level in media samples was not altered after incubation of AP with lactate. These results suggest that the increase of plasma progesterone level in rats during exercise is independent of LH secretion and at least in part is due directly to a stimulatory effect of lactate on the production of ovarian cAMP.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume271
Issue number5 34-5
StatePublished - Nov 1 1996

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Progesterone
Lactic Acid
Anterior Pituitary Gland
Jugular Veins
1-Methyl-3-isobutylxanthine
Femoral Vein
Follicle Stimulating Hormone
Gonadotropin-Releasing Hormone
Cyclic AMP
Ovary
Swine
Glucose

Keywords

  • adenosine 3',5'-cyclic monophosphate
  • exercise
  • leuteinizing hormone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Cite this

Lu, S. S., Lau, C. P., Tung, Y. F., Huang, S. W., Chen, Y. H., Shih, H. C., ... Wang, P. S. (1996). Lactate stimulates progesterone secretion via an increase in cAMP production in exercised female rats. American Journal of Physiology - Endocrinology and Metabolism, 271(5 34-5).

Lactate stimulates progesterone secretion via an increase in cAMP production in exercised female rats. / Lu, Shin Shan; Lau, Chin Pang; Tung, Yuh Fan; Huang, Seng Wong; Chen, Yen Hao; Shih, Hsi Chang; Tsai, Shiow Chwen; Lu, Chien Chen; Wang, Shyi Wu; Chen, Jing Jong; Chien, Eillen Jea; Chien, Chau Heng; Wang, Paulus S.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 271, No. 5 34-5, 01.11.1996.

Research output: Contribution to journalArticle

Lu, SS, Lau, CP, Tung, YF, Huang, SW, Chen, YH, Shih, HC, Tsai, SC, Lu, CC, Wang, SW, Chen, JJ, Chien, EJ, Chien, CH & Wang, PS 1996, 'Lactate stimulates progesterone secretion via an increase in cAMP production in exercised female rats', American Journal of Physiology - Endocrinology and Metabolism, vol. 271, no. 5 34-5.
Lu, Shin Shan ; Lau, Chin Pang ; Tung, Yuh Fan ; Huang, Seng Wong ; Chen, Yen Hao ; Shih, Hsi Chang ; Tsai, Shiow Chwen ; Lu, Chien Chen ; Wang, Shyi Wu ; Chen, Jing Jong ; Chien, Eillen Jea ; Chien, Chau Heng ; Wang, Paulus S. / Lactate stimulates progesterone secretion via an increase in cAMP production in exercised female rats. In: American Journal of Physiology - Endocrinology and Metabolism. 1996 ; Vol. 271, No. 5 34-5.
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abstract = "The effect of exercise on the production of ovarian progesterone was examined in female rats. During in vivo experiments, diestrous rats were catheterized via the right jugular vein (RJV), and blood samples were collected before and after 10, 15, 30, and 60 min of swimming. In addition, blood samples were collected from the RJV before and 2, 5, 10, 15, 30, 60, and 120 min after 10 min of infusion of lactate (13 mg · kg-1 · min-1) through the left femoral vein. To explore if lactate modulates progesterone secretion by acting directly on rat ovary or on anterior pituitary gland (AP), an in vitro experiment that mimicked the in vivo condition was performed. The ovarian tissue was challenged with lactate (0.01-10 mM) or porcine follicle-stimulating hormone (1 μg/ml) and 3-isobutyl-1- methylxanthine (1 mM) for 60 min, and the AP was challenged with lactate ranging from 0.1 to 10 mM or 10 nM gonadotropin-releasing hormone for 30 min. The postexercise levels of plasma glucose, lactate, and progesterone at 10, 15, and 30 min were significantly higher than the corresponding basal levels. Plasma luteinizing hormome (LH) did not change after exercise. An elevation of plasmal lactate and progesterone was found at 15 and 30 min subsequent to 10 min of infusion of lactate. Lactate ranging from 0.01 to 10 mM significantly increased ovarian adenosine 3',5'-cyclic monophosphate (cAMP) and progesterone production in a dose-dependent manner. LH concentration in plasma was not changed subsequent to lactate infusion. LH level in media samples was not altered after incubation of AP with lactate. These results suggest that the increase of plasma progesterone level in rats during exercise is independent of LH secretion and at least in part is due directly to a stimulatory effect of lactate on the production of ovarian cAMP.",
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