Lactobacillus johnsonii supplementation attenuates respiratory viral infection via metabolic reprogramming and immune cell modulation

W. Fonseca, K. Lucey, S. Jang, K. E. Fujimura, A. Rasky, H. A. Ting, J. Petersen, C. C. Johnson, H. A. Boushey, E. Zoratti, Dennis Randall Ownby, A. M. Levine, K. R. Bobbit, S. V. Lynch, N. W. Lukacs

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Regulation of respiratory mucosal immunity by microbial-derived metabolites has been a proposed mechanism that may provide airway protection. Here we examine the effect of oral Lactobacillus johnsonii supplementation on metabolic and immune response dynamics during respiratory syncytial virus (RSV) infection. L. johnsonii supplementation reduced airway T helper type 2 cytokines and dendritic cell (DC) function, increased regulatory T cells, and was associated with a reprogrammed circulating metabolic environment, including docosahexanoic acid (DHA) enrichment. RSV-infected bone marrow-derived DCs (BMDCs) from L. johnsonii-supplemented mice had altered cytokine secretion, reduced expression of co-stimulatory molecules, and modified CD4+ T-cell cytokines. This was replicated upon co-incubation of wild-type BMDCs with either plasma from L. johnsonii-supplemented mice or DHA. Finally, airway transfer of BMDCs from L. johnsonii-supplemented mice or with wild-type derived BMDCs pretreated with plasma from L. johnsonii-supplemented mice reduced airway pathological responses to infection in recipient animals. Thus L. johnsonii supplementation mediates airway mucosal protection via immunomodulatory metabolites and altered immune function.

Original languageEnglish (US)
Pages (from-to)1569-1580
Number of pages12
JournalMucosal Immunology
Volume10
Issue number6
DOIs
StatePublished - Nov 1 2017

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Virus Diseases
Respiratory Tract Infections
Bone Marrow
Cytokines
Respiratory Syncytial Virus Infections
Mucosal Immunity
CD4 Antigens
Th2 Cells
Acids
Respiratory Syncytial Viruses
Regulatory T-Lymphocytes
Cellular Reprogramming
Lactobacillus johnsonii
Dendritic Cells
T-Lymphocytes
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Lactobacillus johnsonii supplementation attenuates respiratory viral infection via metabolic reprogramming and immune cell modulation. / Fonseca, W.; Lucey, K.; Jang, S.; Fujimura, K. E.; Rasky, A.; Ting, H. A.; Petersen, J.; Johnson, C. C.; Boushey, H. A.; Zoratti, E.; Ownby, Dennis Randall; Levine, A. M.; Bobbit, K. R.; Lynch, S. V.; Lukacs, N. W.

In: Mucosal Immunology, Vol. 10, No. 6, 01.11.2017, p. 1569-1580.

Research output: Contribution to journalArticle

Fonseca, W, Lucey, K, Jang, S, Fujimura, KE, Rasky, A, Ting, HA, Petersen, J, Johnson, CC, Boushey, HA, Zoratti, E, Ownby, DR, Levine, AM, Bobbit, KR, Lynch, SV & Lukacs, NW 2017, 'Lactobacillus johnsonii supplementation attenuates respiratory viral infection via metabolic reprogramming and immune cell modulation', Mucosal Immunology, vol. 10, no. 6, pp. 1569-1580. https://doi.org/10.1038/mi.2017.13
Fonseca, W. ; Lucey, K. ; Jang, S. ; Fujimura, K. E. ; Rasky, A. ; Ting, H. A. ; Petersen, J. ; Johnson, C. C. ; Boushey, H. A. ; Zoratti, E. ; Ownby, Dennis Randall ; Levine, A. M. ; Bobbit, K. R. ; Lynch, S. V. ; Lukacs, N. W. / Lactobacillus johnsonii supplementation attenuates respiratory viral infection via metabolic reprogramming and immune cell modulation. In: Mucosal Immunology. 2017 ; Vol. 10, No. 6. pp. 1569-1580.
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abstract = "Regulation of respiratory mucosal immunity by microbial-derived metabolites has been a proposed mechanism that may provide airway protection. Here we examine the effect of oral Lactobacillus johnsonii supplementation on metabolic and immune response dynamics during respiratory syncytial virus (RSV) infection. L. johnsonii supplementation reduced airway T helper type 2 cytokines and dendritic cell (DC) function, increased regulatory T cells, and was associated with a reprogrammed circulating metabolic environment, including docosahexanoic acid (DHA) enrichment. RSV-infected bone marrow-derived DCs (BMDCs) from L. johnsonii-supplemented mice had altered cytokine secretion, reduced expression of co-stimulatory molecules, and modified CD4+ T-cell cytokines. This was replicated upon co-incubation of wild-type BMDCs with either plasma from L. johnsonii-supplemented mice or DHA. Finally, airway transfer of BMDCs from L. johnsonii-supplemented mice or with wild-type derived BMDCs pretreated with plasma from L. johnsonii-supplemented mice reduced airway pathological responses to infection in recipient animals. Thus L. johnsonii supplementation mediates airway mucosal protection via immunomodulatory metabolites and altered immune function.",
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