Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma

Vyomesh Patel, Kay Aldridge, John F. Ensley, Edward Odell, Andrea Boyd, Judith Jones, Silvio Gutkind, William Andrew Yeudall

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the γ2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed over-expression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Immunostaining of HNSCC cells derived from primary (HN4) and metastatic (HN 12) tumors indicated elevated levels of endogenous laminin-γ2 protein. Furthermore, HNSCC tissues demonstrated strong laminin-γ2 staining, particularly in the peripheral basaloid cells of tumor islands at the invasion front. In contrast, only minimal staining of laminin-γ2 was detected in basal cells of the normal epithelium. The data indicate that laminin-γ2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy.

Original languageEnglish (US)
Pages (from-to)583-588
Number of pages6
JournalInternational Journal of Cancer
Volume99
Issue number4
DOIs
StatePublished - Jun 1 2002

Fingerprint

Laminin
RNA
Staining and Labeling
Islet Cell Adenoma
Carcinoma
Cell Line
Mouth Mucosa
Tumor Cell Line
Keratinocytes
Northern Blotting
Neoplasms
Colon
Neck
Epithelium
Epithelial Cells
Head
Carcinoma, squamous cell of head and neck
Gene Expression
Polymerase Chain Reaction
DNA

Keywords

  • Biomarker
  • Differential display
  • Laminin
  • Oral cancer
  • Tumor progression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma. / Patel, Vyomesh; Aldridge, Kay; Ensley, John F.; Odell, Edward; Boyd, Andrea; Jones, Judith; Gutkind, Silvio; Yeudall, William Andrew.

In: International Journal of Cancer, Vol. 99, No. 4, 01.06.2002, p. 583-588.

Research output: Contribution to journalArticle

Patel, V, Aldridge, K, Ensley, JF, Odell, E, Boyd, A, Jones, J, Gutkind, S & Yeudall, WA 2002, 'Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma', International Journal of Cancer, vol. 99, no. 4, pp. 583-588. https://doi.org/10.1002/ijc.10403
Patel V, Aldridge K, Ensley JF, Odell E, Boyd A, Jones J et al. Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma. International Journal of Cancer. 2002 Jun 1;99(4):583-588. https://doi.org/10.1002/ijc.10403
Patel, Vyomesh ; Aldridge, Kay ; Ensley, John F. ; Odell, Edward ; Boyd, Andrea ; Jones, Judith ; Gutkind, Silvio ; Yeudall, William Andrew. / Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma. In: International Journal of Cancer. 2002 ; Vol. 99, No. 4. pp. 583-588.
@article{501f079a203e48549631cf4d2a781ac5,
title = "Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma",
abstract = "To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the γ2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed over-expression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Immunostaining of HNSCC cells derived from primary (HN4) and metastatic (HN 12) tumors indicated elevated levels of endogenous laminin-γ2 protein. Furthermore, HNSCC tissues demonstrated strong laminin-γ2 staining, particularly in the peripheral basaloid cells of tumor islands at the invasion front. In contrast, only minimal staining of laminin-γ2 was detected in basal cells of the normal epithelium. The data indicate that laminin-γ2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy.",
keywords = "Biomarker, Differential display, Laminin, Oral cancer, Tumor progression",
author = "Vyomesh Patel and Kay Aldridge and Ensley, {John F.} and Edward Odell and Andrea Boyd and Judith Jones and Silvio Gutkind and Yeudall, {William Andrew}",
year = "2002",
month = "6",
day = "1",
doi = "10.1002/ijc.10403",
language = "English (US)",
volume = "99",
pages = "583--588",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma

AU - Patel, Vyomesh

AU - Aldridge, Kay

AU - Ensley, John F.

AU - Odell, Edward

AU - Boyd, Andrea

AU - Jones, Judith

AU - Gutkind, Silvio

AU - Yeudall, William Andrew

PY - 2002/6/1

Y1 - 2002/6/1

N2 - To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the γ2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed over-expression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Immunostaining of HNSCC cells derived from primary (HN4) and metastatic (HN 12) tumors indicated elevated levels of endogenous laminin-γ2 protein. Furthermore, HNSCC tissues demonstrated strong laminin-γ2 staining, particularly in the peripheral basaloid cells of tumor islands at the invasion front. In contrast, only minimal staining of laminin-γ2 was detected in basal cells of the normal epithelium. The data indicate that laminin-γ2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy.

AB - To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the γ2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed over-expression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Immunostaining of HNSCC cells derived from primary (HN4) and metastatic (HN 12) tumors indicated elevated levels of endogenous laminin-γ2 protein. Furthermore, HNSCC tissues demonstrated strong laminin-γ2 staining, particularly in the peripheral basaloid cells of tumor islands at the invasion front. In contrast, only minimal staining of laminin-γ2 was detected in basal cells of the normal epithelium. The data indicate that laminin-γ2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy.

KW - Biomarker

KW - Differential display

KW - Laminin

KW - Oral cancer

KW - Tumor progression

UR - http://www.scopus.com/inward/record.url?scp=0036591757&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036591757&partnerID=8YFLogxK

U2 - 10.1002/ijc.10403

DO - 10.1002/ijc.10403

M3 - Article

C2 - 11992550

AN - SCOPUS:0036591757

VL - 99

SP - 583

EP - 588

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -