Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma

Vyomesh Patel, Kay Aldridge, John F. Ensley, Edward Odell, Andrea Boyd, Judith Jones, Silvio Gutkind, W. Andrew Yeudall

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

To identify molecular markers for the progression of head-and-neck squamous cell carcinoma (HNSCC), we used RNA arbitrarily primed (RAP) PCR to determine the qualitative and quantitative differences in gene expression between normal epithelial cells, those derived from dysplastic oral mucosa and invasive and metastatic HNSCC. Three differentially expressed DNA fragments (RAP20, RAP21, RAP26) that were upregulated in a tumor cell line (T45) were identified as being regions of the γ2 subunit of human laminin-5. Northern blot analysis of total cellular RNA revealed over-expression of these transcripts in 6 of 7 HNSCC cell lines compared with normal epidermal keratinocytes. In contrast, no differences were observed in HeLa (cervical carcinoma) or HCT116 (colon carcinoma) cells. Immunostaining of HNSCC cells derived from primary (HN4) and metastatic (HN 12) tumors indicated elevated levels of endogenous laminin-γ2 protein. Furthermore, HNSCC tissues demonstrated strong laminin-γ2 staining, particularly in the peripheral basaloid cells of tumor islands at the invasion front. In contrast, only minimal staining of laminin-γ2 was detected in basal cells of the normal epithelium. The data indicate that laminin-γ2 is frequently overexpressed in HNSCCs and derivative cell lines and that its overexpression is likely to be useful as a marker of head-and-neck squamous malignancy.

Original languageEnglish (US)
Pages (from-to)583-588
Number of pages6
JournalInternational Journal of Cancer
Volume99
Issue number4
DOIs
StatePublished - Jun 1 2002
Externally publishedYes

Keywords

  • Biomarker
  • Differential display
  • Laminin
  • Oral cancer
  • Tumor progression

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Patel, V., Aldridge, K., Ensley, J. F., Odell, E., Boyd, A., Jones, J., Gutkind, S., & Yeudall, W. A. (2002). Laminin-γ2 overexpression in head-and-neck squamous cell carcinoma. International Journal of Cancer, 99(4), 583-588. https://doi.org/10.1002/ijc.10403