Lanthanum potentiation of the vascular response to a protein kinase C activator in genetically hypertensive rats

Jeffrey Raval, Irving G. Joshua, R Clinton Webb, David F. Bohr

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effects of lanthanum on the contraction induced by the protein kinase C activator, 12-0-tetradecanoylphorbol-13-ac-etate (TPA) were studied in femoral artery rings from stroke- prone, spontaneously hypertensive rats (SHRSP) and normo- tensive Wistar-Kyoto rats (WKY). When exposed to a calcium- free buffer containing 1 mmol/l EGTA, the femoral artery rings from SHRSP and WKY, pre-contracted with TPA (10-6mol/l), relaxed by 52 and 24%, respectively. Treatment of the rings in this calcium-free buffer with 2.6 mmol/l lanthanum significantly potentiated the TPA-induced contractions in vascular rings from WKY (49%) and SHRSP (136%). Potentiation by lanthanum of the TPA-induced contraction in the absence of extracellular calcium suggests that this cation is acting intracellularly to increase protein kinase C activity. The in-creased vascular responsiveness of SHRSP to lanthanum may reflect an abnormality in protein kinase C activation in vascular smooth muscle of genetically hypertensive rats.

Original languageEnglish (US)
Pages (from-to)S146-S147
JournalJournal of Hypertension, Supplement
Volume7
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Lanthanum
Protein Kinase C
Blood Vessels
Inbred WKY Rats
Femoral Artery
Calcium
Buffers
Egtazic Acid
Inbred SHR Rats
Vascular Smooth Muscle
Cations
Stroke

Keywords

  • Calcium
  • Femoral artery
  • Genetic hypertension
  • Lanthanum
  • Protein kinase C activators

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Lanthanum potentiation of the vascular response to a protein kinase C activator in genetically hypertensive rats. / Raval, Jeffrey; Joshua, Irving G.; Webb, R Clinton; Bohr, David F.

In: Journal of Hypertension, Supplement, Vol. 7, 01.01.1989, p. S146-S147.

Research output: Contribution to journalArticle

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AU - Joshua, Irving G.

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AB - The effects of lanthanum on the contraction induced by the protein kinase C activator, 12-0-tetradecanoylphorbol-13-ac-etate (TPA) were studied in femoral artery rings from stroke- prone, spontaneously hypertensive rats (SHRSP) and normo- tensive Wistar-Kyoto rats (WKY). When exposed to a calcium- free buffer containing 1 mmol/l EGTA, the femoral artery rings from SHRSP and WKY, pre-contracted with TPA (10-6mol/l), relaxed by 52 and 24%, respectively. Treatment of the rings in this calcium-free buffer with 2.6 mmol/l lanthanum significantly potentiated the TPA-induced contractions in vascular rings from WKY (49%) and SHRSP (136%). Potentiation by lanthanum of the TPA-induced contraction in the absence of extracellular calcium suggests that this cation is acting intracellularly to increase protein kinase C activity. The in-creased vascular responsiveness of SHRSP to lanthanum may reflect an abnormality in protein kinase C activation in vascular smooth muscle of genetically hypertensive rats.

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