Laser-induced thrombosis in zebrafish

Pudur Jagadeeswaran, Maira Carrillo, Uvaraj P Radhakrishnan, Surendra Kumar Rajpurohit, Seongcheol Kim

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In the event of injury to the vasculature in vertebrate organisms bleeding is stopped by a defense mechanism called hemostasis. Even though biochemical studies characterized a number of factors, classical genetic methods have not been applied to study hemostasis. We introduced zebrafish as an animal model to study genetics of hemostasis. To conduct genetic studies of hemostasis, we required a global screening method to address all the factors of hemostasis such as those present in plasma, in platelets or those present in the endothelium. Therefore, we developed a global laser induced thrombosis method which can assay all these components. In this paper, we describe the principle of this method as well as provide the detailed protocol so this could be used as a screening tool to measure hemostasis in any laboratory.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalMethods in Cell Biology
Volume101
DOIs
StatePublished - 2011

Keywords

  • Animals
  • Disease Models, Animal
  • Hemostasis
  • Humans
  • Lasers
  • Thrombosis
  • Zebrafish

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