Lck couples Shc to TCR signaling

Atsuki Fukushima, Yasue Hatanaka, Jing Wen Chang, Masako Takamatsu, Nagendra Singh, Makio Iwashima

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Recent genetic evidence demonstrated that Shc is a critical molecule for T cell activation and differentiation. However, how Shc is coupled to the T cell antigen receptor (TCR) has not been clearly characterized. Here we report that the tyrosine kinase Lck functions as a connecting molecule for TCR and Shc. Lck plays a critical role in TCR signal transduction by phosphorylating the immuno-receptor tyrosine based activation motif (ITAM). Our data shows that the PTB domain of Shc binds the SH2/3 domains of Lck in a phosphotyrosine-independent manner. Inhibition of the Lck/Shc interaction led to the loss of IL-2 promoter activation, confirming that the role of Shc in IL-2 production requires its interaction with Lck. Together, the data show that Shc is connected to the activated TCR via direct interaction with Lck.

Original languageEnglish (US)
Pages (from-to)1182-1189
Number of pages8
JournalCellular Signalling
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2006

Keywords

  • LAT
  • Lck
  • PTB
  • SH2
  • SH3
  • Shc
  • TCR
  • ZAP-70

ASJC Scopus subject areas

  • Cell Biology

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    Fukushima, A., Hatanaka, Y., Chang, J. W., Takamatsu, M., Singh, N., & Iwashima, M. (2006). Lck couples Shc to TCR signaling. Cellular Signalling, 18(8), 1182-1189. https://doi.org/10.1016/j.cellsig.2005.09.008