Lck couples Shc to TCR signaling

Atsuki Fukushima, Yasue Hatanaka, Jing Wen Chang, Masako Takamatsu, Nagendra Singh, Makio Iwashima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Recent genetic evidence demonstrated that Shc is a critical molecule for T cell activation and differentiation. However, how Shc is coupled to the T cell antigen receptor (TCR) has not been clearly characterized. Here we report that the tyrosine kinase Lck functions as a connecting molecule for TCR and Shc. Lck plays a critical role in TCR signal transduction by phosphorylating the immuno-receptor tyrosine based activation motif (ITAM). Our data shows that the PTB domain of Shc binds the SH2/3 domains of Lck in a phosphotyrosine-independent manner. Inhibition of the Lck/Shc interaction led to the loss of IL-2 promoter activation, confirming that the role of Shc in IL-2 production requires its interaction with Lck. Together, the data show that Shc is connected to the activated TCR via direct interaction with Lck.

Original languageEnglish (US)
Pages (from-to)1182-1189
Number of pages8
JournalCellular Signalling
Volume18
Issue number8
DOIs
StatePublished - Aug 1 2006

Fingerprint

T-Cell Antigen Receptor
Interleukin-2
Immunoreceptor Tyrosine-Based Activation Motif
Phosphotyrosine
src Homology Domains
Protein-Tyrosine Kinases
Cell Differentiation
Signal Transduction
T-Lymphocytes

Keywords

  • LAT
  • Lck
  • PTB
  • SH2
  • SH3
  • Shc
  • TCR
  • ZAP-70

ASJC Scopus subject areas

  • Cell Biology

Cite this

Fukushima, A., Hatanaka, Y., Chang, J. W., Takamatsu, M., Singh, N., & Iwashima, M. (2006). Lck couples Shc to TCR signaling. Cellular Signalling, 18(8), 1182-1189. https://doi.org/10.1016/j.cellsig.2005.09.008

Lck couples Shc to TCR signaling. / Fukushima, Atsuki; Hatanaka, Yasue; Chang, Jing Wen; Takamatsu, Masako; Singh, Nagendra; Iwashima, Makio.

In: Cellular Signalling, Vol. 18, No. 8, 01.08.2006, p. 1182-1189.

Research output: Contribution to journalArticle

Fukushima, A, Hatanaka, Y, Chang, JW, Takamatsu, M, Singh, N & Iwashima, M 2006, 'Lck couples Shc to TCR signaling', Cellular Signalling, vol. 18, no. 8, pp. 1182-1189. https://doi.org/10.1016/j.cellsig.2005.09.008
Fukushima A, Hatanaka Y, Chang JW, Takamatsu M, Singh N, Iwashima M. Lck couples Shc to TCR signaling. Cellular Signalling. 2006 Aug 1;18(8):1182-1189. https://doi.org/10.1016/j.cellsig.2005.09.008
Fukushima, Atsuki ; Hatanaka, Yasue ; Chang, Jing Wen ; Takamatsu, Masako ; Singh, Nagendra ; Iwashima, Makio. / Lck couples Shc to TCR signaling. In: Cellular Signalling. 2006 ; Vol. 18, No. 8. pp. 1182-1189.
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