Lead and cadmium co-exposure mediated toxic insults on hepatic steroid metabolism and antioxidant system of adult male rats

Chirayukumar D Pandya, Prakash P. Pillai, Sarita S. Gupta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The redox status and steroid metabolism of liver of adult male rat exposed to lead (Pb) and cadmium (Cd) either alone or in co-exposure (0.025 mg/kg body weight intraperitoneally/15 days) was studied. Pb and Cd significantly accumulated in the liver. The activity of steroid metabolizing enzymes 17-βhydroxysteroid oxidoreductase and uridine diphosphate- glucuronyltransferase were decreased in experimental animals. 17-β-Hydroxysteroid dehydrogenase was reduced to 33%, 38%, and 24% on treatment of Pb, Cd, and co-exposure (Pb+Cd). Furthermore, the activity of uridine diphosphate-glucuronosyltransferase was significantly reduced to 27% (Pb exposure), 36% (Cd exposure), and 25% (co-exposure of Pb+Cd). Cd exposure exhibited more toxic effect than Pb, while co-exposure demonstrated the least. The activities of antioxidant enzymes, superoxide dismutase, catalase, glutathione reductase, and glucose-6-phosphate dehydrogenase decreased and glutathione peroxidase increased in mitochondrial and post-mitochondrial fractions. The level of lipid peroxidation increased, and cellular glutathione concentration decreased. Hepatic DNA was decreased, whereas RNA content and the activity of alanine transaminase remained unchanged. Histological studies revealed that only Cd-exposed groups exhibited cytotoxic effect. These results suggest that when Pb and Cd are present together in similar concentrations, they exhibited relatively decreased toxic effect when compared to lead and cadmium in isolation with regard to decreased steroid metabolizing and antioxidant enzyme activities. This seems that the toxic effect of these metals is antagonized by co-exposure due to possible competition amongst Pb and Cd for hepatic accumulation.

Original languageEnglish (US)
Pages (from-to)307-317
Number of pages11
JournalBiological Trace Element Research
Volume134
Issue number3
DOIs
StatePublished - Jun 1 2010

Fingerprint

Poisons
Cadmium
Metabolism
Rats
Antioxidants
Steroids
Liver
Glucuronosyltransferase
Uridine Diphosphate
Lead
Enzymes
Hydroxysteroids
Glutathione Reductase
Glucosephosphate Dehydrogenase
Enzyme activity
Glutathione Peroxidase
Alanine Transaminase
Catalase
Lipid Peroxidation
Superoxide Dismutase

Keywords

  • Antioxidant enzyme
  • Cd
  • Pb
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Inorganic Chemistry
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism

Cite this

Lead and cadmium co-exposure mediated toxic insults on hepatic steroid metabolism and antioxidant system of adult male rats. / Pandya, Chirayukumar D; Pillai, Prakash P.; Gupta, Sarita S.

In: Biological Trace Element Research, Vol. 134, No. 3, 01.06.2010, p. 307-317.

Research output: Contribution to journalArticle

Pandya, Chirayukumar D ; Pillai, Prakash P. ; Gupta, Sarita S. / Lead and cadmium co-exposure mediated toxic insults on hepatic steroid metabolism and antioxidant system of adult male rats. In: Biological Trace Element Research. 2010 ; Vol. 134, No. 3. pp. 307-317.
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