Lectins as Probes to Pneumocystis carinii Surface Glycocomplexes

Jamie Ann De Stefano, M. T. CUSHION, L. S. TRINKLE, P. D. WALZER

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The binding characteristics of a panel of commercially available FITC‐conjugated lectins to Pneumocystis carinii (Pc) were assessed by fluorescence microscopy and flow cytometry. Rat Pc obtained from infecteding homogenates were incubated with FTTC‐conjugated lectins in a series of concentrations, counlerstained with propidium iodide, and analyzed for percent fluorescence and fluorescence intensity. All organisms bound concanavalin A and Wisteria floribunda agglutinin, 2 representatives of the glucose/mannose‐binding group. From the lectin group specific for N‐acctylglucosamine, Pc reacted more strongly with wheat germ agglutinin than with Solanum tuberosum agglutinin or Griffonia simpiicifolia II lectin. Pneumocystis treated with lectins specific for N‐acetyl‐D‐galactosamine and galactose exhibited much variation; the cells reacted moderately well to soybean agglutinin and less to Bauhinia purpurea, Madura pomifera and Dolichos biflorus agglutinins and Giffonia simpiicifolia Hectin. Arachis hypogaea agglutinin, Viscum album agglutinin and Griffon'ui simpiicifolia I—β Section had not effect. The organisms reacted weakly with Ulex europeus I agglutinin which is specific for fucose and did not react with Limax ftavus lectin, which is specific for sialic acid. Competitive inhibition studies using relevant carbohydrates were performed to indicate that the positive reactions were specific. These studies should help to elucidate the mechanisms of attachment and pathogenesis of this organism.

Original languageEnglish (US)
Pages (from-to)65S-66S
JournalThe Journal of Protozoology
Volume36
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Pneumocystis carinii
Lectins
Agglutinins
Bauhinia
Griffonia
Viscum album
Fluorescence
Pneumocystis
Ulex
Wheat Germ Agglutinins
Fucose
Indonesia
Propidium
N-Acetylneuraminic Acid
Concanavalin A
Galactose
Fluorescence Microscopy
Flow Cytometry
Carbohydrates
Glucose

ASJC Scopus subject areas

  • Parasitology

Cite this

Lectins as Probes to Pneumocystis carinii Surface Glycocomplexes. / De Stefano, Jamie Ann; CUSHION, M. T.; TRINKLE, L. S.; WALZER, P. D.

In: The Journal of Protozoology, Vol. 36, No. 1, 01.01.1989, p. 65S-66S.

Research output: Contribution to journalArticle

De Stefano, Jamie Ann ; CUSHION, M. T. ; TRINKLE, L. S. ; WALZER, P. D. / Lectins as Probes to Pneumocystis carinii Surface Glycocomplexes. In: The Journal of Protozoology. 1989 ; Vol. 36, No. 1. pp. 65S-66S.
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