Lentivector expressing HBsAG and immunoglobulin Fc fusion antigen induces potent immune responses and results in seroconversion in HBsAG transgenic mice

Yuan Hong, Yibing Peng, Michael Mi, Haiyan Xiao, David H Munn, Gui Qiang Wang, Yukai He

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Even though hepatitis B virus(HBV) vaccines effectively prevent new cases of HBV infection, with approximately 350 million patients worldwide, chronic HBV infection remains a major health problem because of the associated complications (such as liver cirrhosis and hepatocellular carcinoma) and the limited treatment options. Immunotherapy has the potential to effectively control HBV replication. In this current study, we found that recombinant lentivectors could induce potent HBV surface antigen (HBsAg) specific T cell responses and humoral immune responses. Tagging the HBsAg with immunoglobulin Fc fragment further substantially increased the HBsAg specific immune responses. Remarkably, the HBSFc-lv lentivector could effectively break immune tolerance and induce potent HBsAg specific adaptive immune responses in HBsAg transgenic (Tg) mice with low serum level of HBsAg. More importantly, the induction of HBsAg specific immune responses in Tg mice accompanied seroconversion from HBsAg to anti-HBsAg antibody (anti-HBsAb). Our study demonstrated the potential of utilizing lentivector to treat chronic HBV infection following reduction of viral load with antiviral drug therapy.

Original languageEnglish (US)
Pages (from-to)3909-3916
Number of pages8
JournalVaccine
Volume29
Issue number22
DOIs
StatePublished - May 17 2011

Keywords

  • Chronic HBV infection
  • Genetic immunization
  • Hepatitis B virus
  • Immunotherapy
  • Lentivector
  • Vaccines

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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