Leptin action is modified by an interaction between dietary fat content and ambient temperature

Andrea L. Haltiner, Tiffany D. Mitchell, Ruth Babette Harris

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mice adapted to a high-fat diet are reported to be leptin resistant; however, we previously reported that mice fed a high-fat (HF) diet and housed at 23°C remained sensitive to peripheral leptin and specifically lost body fat. This study tested whether leptin action was impaired by a combination of elevated environmental temperature and a HF diet. Male C57BL/6 mice were adapted to low-fat (LF) or HF diet from 10 days of age and were housed at 27°C from 28 days of age. From 35 days of age, baseline food intake and body weight were recorded for 1 wk and then mice on each diet were infused with 10 μg leptin/day or PBS from an intraperitoneal miniosmotic pump for 13 days. HF-fed mice had a higher energy intake than LF-fed mice and were heavier but not fatter. Serum leptin was lower in PBS-infused HF- than LF-fed mice. Leptin significantly inhibited energy intake of both LF-fed and HF-fed mice, and this was associated with a significant increase in hypothalamic long-form leptin receptors with no change in short-form leptin receptor or brown fat uncoupling protein-1 mRNA expression. Leptin significantly inhibited weight gain in both LF- and HF-fed mice but reduced the percentage of body fat mass only in LF-fed mice. The percentage of lean and fat tissue in HF-fed mice did not change, implying that overall growth had been inhibited. These results suggest that dietary fat modifies the mechanisms responsible for leptin-induced changes in body fat content and that those in HF-fed mice are sensitive to environmental temperature.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume287
Issue number5 56-5
DOIs
StatePublished - Nov 1 2004
Externally publishedYes

Fingerprint

Dietary Fats
Leptin
Fats
Temperature
High Fat Diet
Adipose Tissue
Leptin Receptors
Energy Intake
Brown Adipose Tissue
Inbred C57BL Mouse
Weight Gain

Keywords

  • Growth
  • Mice
  • Peripheral leptin

ASJC Scopus subject areas

  • Physiology

Cite this

Leptin action is modified by an interaction between dietary fat content and ambient temperature. / Haltiner, Andrea L.; Mitchell, Tiffany D.; Harris, Ruth Babette.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 287, No. 5 56-5, 01.11.2004.

Research output: Contribution to journalArticle

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