Leptin increases adult hippocampal neurogenesis in vivo and in vitro

Jacob C. Garza, Ming Guo, Wei Zhang, Xinyun Lu

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Leptin, an adipose-derived hormone, has been implicated in several physiological processes involving the hippocampus. However, the role of leptin in adult hippocampal neurogenesis remains unknown. Here we show that leptin regulates neurogenesis in the dentate gyrus of adult mice as well as in cultured adult hippocampal progenitor cells. Chronic administration of leptin to adult mice increased cell proliferation without significant effects on the differentiation and the survival of newly proliferated cells in the dentate gyrus. The expression of the long form leptin receptor, LepRb, was detected in hippocampal progenitor cells by reverse transcription-PCR and immunohistochemistry. Leptin treatment also increased proliferation of cultured adult hippocampal progenitor cells. Analysis of signal transduction pathways revealed that leptin stimulated phosphorylation of Akt and STAT3 but not ERK1/2. Furthermore, pretreating the cells with specific inhibitors of Akt or STAT3 attenuated leptin-induced cell proliferation in a dose-dependent manner. Taken together, our results support a role for leptin in adult hippocampal neurogenesis and suggest the involvement of the Akt and STAT3 signaling pathways in mediating the actions of leptin on neurogenesis.

Original languageEnglish (US)
Pages (from-to)18238-18247
Number of pages10
JournalJournal of Biological Chemistry
Volume283
Issue number26
DOIs
StatePublished - Jun 27 2008

Fingerprint

Neurogenesis
Leptin
Stem Cells
Dentate Gyrus
Cell proliferation
Cell Proliferation
Physiological Phenomena
In Vitro Techniques
Leptin Receptors
Signal transduction
Phosphorylation
Transcription
Reverse Transcription
Signal Transduction
Hippocampus
Immunohistochemistry
Hormones
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Leptin increases adult hippocampal neurogenesis in vivo and in vitro. / Garza, Jacob C.; Guo, Ming; Zhang, Wei; Lu, Xinyun.

In: Journal of Biological Chemistry, Vol. 283, No. 26, 27.06.2008, p. 18238-18247.

Research output: Contribution to journalArticle

Garza, Jacob C. ; Guo, Ming ; Zhang, Wei ; Lu, Xinyun. / Leptin increases adult hippocampal neurogenesis in vivo and in vitro. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 26. pp. 18238-18247.
@article{b45980f2a39f4b118117e06e7b46d238,
title = "Leptin increases adult hippocampal neurogenesis in vivo and in vitro",
abstract = "Leptin, an adipose-derived hormone, has been implicated in several physiological processes involving the hippocampus. However, the role of leptin in adult hippocampal neurogenesis remains unknown. Here we show that leptin regulates neurogenesis in the dentate gyrus of adult mice as well as in cultured adult hippocampal progenitor cells. Chronic administration of leptin to adult mice increased cell proliferation without significant effects on the differentiation and the survival of newly proliferated cells in the dentate gyrus. The expression of the long form leptin receptor, LepRb, was detected in hippocampal progenitor cells by reverse transcription-PCR and immunohistochemistry. Leptin treatment also increased proliferation of cultured adult hippocampal progenitor cells. Analysis of signal transduction pathways revealed that leptin stimulated phosphorylation of Akt and STAT3 but not ERK1/2. Furthermore, pretreating the cells with specific inhibitors of Akt or STAT3 attenuated leptin-induced cell proliferation in a dose-dependent manner. Taken together, our results support a role for leptin in adult hippocampal neurogenesis and suggest the involvement of the Akt and STAT3 signaling pathways in mediating the actions of leptin on neurogenesis.",
author = "Garza, {Jacob C.} and Ming Guo and Wei Zhang and Xinyun Lu",
year = "2008",
month = "6",
day = "27",
doi = "10.1074/jbc.M800053200",
language = "English (US)",
volume = "283",
pages = "18238--18247",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "26",

}

TY - JOUR

T1 - Leptin increases adult hippocampal neurogenesis in vivo and in vitro

AU - Garza, Jacob C.

AU - Guo, Ming

AU - Zhang, Wei

AU - Lu, Xinyun

PY - 2008/6/27

Y1 - 2008/6/27

N2 - Leptin, an adipose-derived hormone, has been implicated in several physiological processes involving the hippocampus. However, the role of leptin in adult hippocampal neurogenesis remains unknown. Here we show that leptin regulates neurogenesis in the dentate gyrus of adult mice as well as in cultured adult hippocampal progenitor cells. Chronic administration of leptin to adult mice increased cell proliferation without significant effects on the differentiation and the survival of newly proliferated cells in the dentate gyrus. The expression of the long form leptin receptor, LepRb, was detected in hippocampal progenitor cells by reverse transcription-PCR and immunohistochemistry. Leptin treatment also increased proliferation of cultured adult hippocampal progenitor cells. Analysis of signal transduction pathways revealed that leptin stimulated phosphorylation of Akt and STAT3 but not ERK1/2. Furthermore, pretreating the cells with specific inhibitors of Akt or STAT3 attenuated leptin-induced cell proliferation in a dose-dependent manner. Taken together, our results support a role for leptin in adult hippocampal neurogenesis and suggest the involvement of the Akt and STAT3 signaling pathways in mediating the actions of leptin on neurogenesis.

AB - Leptin, an adipose-derived hormone, has been implicated in several physiological processes involving the hippocampus. However, the role of leptin in adult hippocampal neurogenesis remains unknown. Here we show that leptin regulates neurogenesis in the dentate gyrus of adult mice as well as in cultured adult hippocampal progenitor cells. Chronic administration of leptin to adult mice increased cell proliferation without significant effects on the differentiation and the survival of newly proliferated cells in the dentate gyrus. The expression of the long form leptin receptor, LepRb, was detected in hippocampal progenitor cells by reverse transcription-PCR and immunohistochemistry. Leptin treatment also increased proliferation of cultured adult hippocampal progenitor cells. Analysis of signal transduction pathways revealed that leptin stimulated phosphorylation of Akt and STAT3 but not ERK1/2. Furthermore, pretreating the cells with specific inhibitors of Akt or STAT3 attenuated leptin-induced cell proliferation in a dose-dependent manner. Taken together, our results support a role for leptin in adult hippocampal neurogenesis and suggest the involvement of the Akt and STAT3 signaling pathways in mediating the actions of leptin on neurogenesis.

UR - http://www.scopus.com/inward/record.url?scp=49649118850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=49649118850&partnerID=8YFLogxK

U2 - 10.1074/jbc.M800053200

DO - 10.1074/jbc.M800053200

M3 - Article

VL - 283

SP - 18238

EP - 18247

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 26

ER -