Leptin resistance in mice is determined by gender and duration of exposure to high-fat diet

Ruth Babette Harris, Heather M. Bowen, Tiffany D. Mitchell

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mice fed a high-fat diet are reported to be resistant to peripheral injections of leptin. We previously failed to induce leptin resistance in female mice fed a high-fat diet for 15 weeks. Therefore, we measured the responsiveness to peripheral infusions (10 μg/day) of leptin, and the responsiveness to third ventricle injections of leptin (1 μg) in male and female NIH Swiss mice fed low-fat (10% kcal) or high-fat (45% kcal) diets. Male and female 15-week-old mice that had been fed low- or high-fat diet from 10 days of age lost fat during a 13-day intraperitoneal infusion of leptin and lost weight in response to a single central injection of leptin. Fifteen-week-old male mice fed a high-fat diet for 5 weeks did not lose body fat during a peripheral infusion of leptin and did not lose weight in response to a central injection of leptin. Female mice fed high-fat diet for 5 weeks remained leptin-responsive. Weight loss was achieved without a significant voluntary decrease in food intake, suggesting that both peripherally and centrally administered leptin increases energy expenditure. These results demonstrate that the development of leptin resistance in NIH Swiss mice fed a high-fat diet is dependent upon the gender of the mice and either the duration of exposure to high-fat diet or the age at which the mice are first exposed to the diet.

Original languageEnglish (US)
Pages (from-to)543-555
Number of pages13
JournalPhysiology and Behavior
Volume78
Issue number4-5
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

High Fat Diet
Leptin
Fats
Injections
Parenteral Infusions
Diet
Weights and Measures
Third Ventricle
Energy Metabolism
Adipose Tissue
Weight Loss
Eating

Keywords

  • Body composition
  • Leptin receptors
  • Obesity

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

Cite this

Leptin resistance in mice is determined by gender and duration of exposure to high-fat diet. / Harris, Ruth Babette; Bowen, Heather M.; Mitchell, Tiffany D.

In: Physiology and Behavior, Vol. 78, No. 4-5, 01.01.2003, p. 543-555.

Research output: Contribution to journalArticle

Harris, Ruth Babette ; Bowen, Heather M. ; Mitchell, Tiffany D. / Leptin resistance in mice is determined by gender and duration of exposure to high-fat diet. In: Physiology and Behavior. 2003 ; Vol. 78, No. 4-5. pp. 543-555.
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